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| Status |
Public on Dec 01, 2010 |
| Title |
Genome-wide connection between DNA methylation and DNA replication timing |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by SNP array
|
| Summary |
DNA methylation and DNA replication timing were examined across a variety of human tissues and cell lines, applying microarray-based techniques. The analyses revealed that late-replicating DNA was demethylated compared to the methylation of early-replicating regions.
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| |
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| Overall design |
DNA methylation: Epstein-Barr-Virus (EBV) transformed B-lymphocyte cell lines GM10849, GM12089, GM12092, GM12093, and GM08714 (ICF) (http://ccr.coriell.org/nigms) were cultured in RPMI-1640 supplemented with 15% FCS (Sigma) at 37oC and 5% CO2. Normal BJ foreskin fibroblasts (NHF cells) at different PDs (36)(34) were cultured in 4:1 DMEM : M-199 supplemented with 15% FCS (Sigma) at 37oC and 5% CO2. Replication timing: EBV-transformed female B-lymphocyte cell lines GM12092 and GM12093 were cultured as above, harvested from logarithmic growth cultures, washed once in ice-cold phosphate-buffered saline, immediately fixed in 70-85-95% ethanol at -20oC, stained with propidium iodide, and sorted into G1 and early-S fractions using a MoFlo cell sorter.
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| |
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| Contributor(s) |
Hellman A |
| Citation(s) |
21112978 |
| Submission date |
Oct 27, 2010 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Asaf Hellman |
| E-mail(s) |
asafh@ekmd.huji.ac.il
|
| Phone |
972-2-6758476
|
| Fax |
972-2-6758243
|
| Organization name |
Hebrew University
|
| Department |
Developmental Biology and Cancer Research
|
| Street address |
Hadassah Medical School
|
| City |
Jerusalem |
| ZIP/Postal code |
91120 |
| Country |
Israel |
| |
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| Platforms (3) |
| GPL3718 |
[Mapping250K_Nsp] Affymetrix Mapping 250K Nsp SNP Array |
| GPL3720 |
[Mapping250K_Sty] Affymetrix Mapping 250K Sty2 SNP Array |
| GPL6801 |
[GenomeWideSNP_6] Affymetrix Genome-Wide Human SNP 6.0 Array |
|
| Samples (46)
|
|
| Relations |
| BioProject |
PRJNA131889 |
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