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| Status |
Public on May 01, 2024 |
| Title |
Histone lactylation antagonizes Senescent via facilitating gene-expression reprogramming [RNA-Seq II] |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The hallmark of aging is a remarkable rearrangement of histone modifications, which reflect alterations in the cellular environment. Here, we show that histone lactylation, a novel histone modification that bridges metabolism and epigenetics, plays a crucial role in antagonizing Senescent. The level of histone lactylation is markedly decreased during Senescent but restored following anti-Senescent treatment such as physiological hypoxic conditions and the application of nicotinamide mononucleotide, a potent precursor for NAD+. We describe the genome-wide distribution profile and gene expression network of histone lactylation during Senescent, revealing that histone lactylation prevents the Senescent program by increasing the expression of anti-aging genes and activating related signaling pathways. Moreover, running exercise enhances the level of histone lactylation and reconstructs the cell composition of mouse skeletal muscle, leading to functional improvement. Our data reveal the function of histone lactylation during Senescent and demonstrate that this modification can be used as a novel marker of Senescent, and perhaps even a potential target for aging intervention.
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| Overall design |
RNA-seq data for IMR90 cells during replicative senescence, upon hypoxia, 2-DG, and NMN treatment; RNA-seq data for MEF cells upon NMN and NALA treatment; RNA-seq data for IMR90 cells upon A485 treatment and MS-275 treatment; RNA-seq data for C2C12 cells after LDHA knockdown; RNA-seq data for mouse skeletal muscle after MS-275 injection. CUT&Tag data for IMR90 cells during replicative senescence, upon hypoxia, 2-DG, and NMN treatment. snRNA-seq data for aging, young, sedentary, and running exercise mouse skeletal muscle.
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| Contributor(s) |
Zhang X, Meng F, He J, Tao W |
| Citation missing |
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| Submission date |
Dec 06, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
Wei Tao |
| E-mail(s) |
weitao@pku.edu.cn
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| Phone |
010-62758903
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| Organization name |
Peking University
|
| Department |
School of Life Sciences
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| Lab |
Tao lab
|
| Street address |
No.5 Yiheyuan Road
|
| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (17)
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| This SubSeries is part of SuperSeries: |
| GSE226008 |
Histone lactylation antagonizes senescence via facilitating gene-expression reprogramming. |
|
| Relations |
| BioProject |
PRJNA1049487 |
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