|
| Status |
Public on May 06, 2005 |
| Title |
Gata-1 Knock Down - Wild Type Megakaryocyte Gene Expression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Hematopoietic progenitor cells were isolated from 13.5 day mouse fetal livers by lineage depletion and expanded for three days. Fetal livers were isolated from both wild type and Gata-1 knock embryos. Gata-1 knock embryos contain a deletion of the Gata-1 promoter sequence that results in undetectable levels of Gata-1 protein specifically in the megakaryocyte lineage. Following progenitor outgrowth megakaryocytes were enriched in a differentiation media for three days and isolated on a discontinuous BSA gradient. The resulting megakaryocytes were >90% pure as determined by acetylcholinesterase staining. These cells were lysed in Trizol and the resulting RNA was used for hybridization.
Keywords = Gata-1
Keywords = Megakaryocytes
Keywords: other
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| Contributor(s) |
Muntean AG, Crispino JD |
| Citation(s) |
15860665 |
| Submission date |
Apr 14, 2005 |
| Last update date |
Feb 11, 2019 |
| Contact name |
John Crispino |
| E-mail(s) |
crispino@huggins.bsd.uchicago.edu
|
| Phone |
(773) 834-7457
|
| Organization name |
The University of Chicago
|
| Street address |
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA92071 |
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