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| Status |
Public on Aug 13, 2012 |
| Title |
Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity [gene expression] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We used an unbiased systems biology approach to study the regulation of gene expression in human adipose tissue focusing on inflammation. We show that microRNAs play a major role as regulators of CCL2 production in obesity.
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| Overall design |
Abdominal subcutaneous adipose needle biopsies were obtained from women (n=56) with a wide variation in BMI. From the biopsies we prepared and hybridised biotinylated complementary RNA to GeneChip Human Gene 1.0 ST Arrays (Affymetrix Inc., Santa Clara, CA), labelled RNA to Affymetrix miRNA arrays, and bisulphite converted DNA to Illumina Infinium HumanMethylation27 beadchips using standardised protocols (Affymetrix Inc., Illumina Inc.) . Subsequent analyses of gene expression was performed using the Affymetrix GeneChip Operating Software version 1.4. To allow comparisons of transcript levels between samples, all samples were subjected to an all-probeset scaling-to-target signal of 100.
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| Contributor(s) |
Mejhert N, Arner E, Dahlman I, Arner P |
| Citation(s) |
22688341, 24439387, 24379347, 27346647, 29348496, 31383551 |
| Submission date |
Nov 16, 2010 |
| Last update date |
Sep 03, 2019 |
| Contact name |
Ingrid Dahlman |
| E-mail(s) |
ingrid.dahlman@ki.se
|
| Organization name |
Karolinska
|
| Street address |
Karolinska Huddinge
|
| City |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (56)
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| This SubSeries is part of SuperSeries: |
| GSE25402 |
Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity |
|
| Relations |
| BioProject |
PRJNA142625 |
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