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| Status |
Public on Aug 01, 2011 |
| Title |
Resveratrol induces downregulation of DNA repair genes in MCF7 human breast cancer cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Breast cancer is a worlwide health problem. One of the factors involved in carcinogenesis is diet. Several studies indicate that resveratrol, a polyphenol present in diet, has several antitumor
properties. The purpose of the study is gain an insight of resveratrol effect in cancer by determining the gene expression profile of breast cancer cell lines treated with the compound.
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| Overall design |
MCF7 cell lines were treated for a 48 hour period with two concentrations (150 and 250 uM) of resveratrol. 9 plates were used for each condition. The RNA obtained from 3 plates of the same condition was pooled, processed and hybridized to the Affymetrix Human Gene 1.0 ST array. The arrays were analyzed using Partek Genomics suite software-.
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| Contributor(s) |
León-Galicia I, Diaz-Chavez J, Garcia-Villa E, Uribe-Figueroa L, Hidalgo-Miranda A, Alvarez-Rios E, García-Mena J, Gariglio P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 16, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Laura I Uribe |
| E-mail(s) |
luribe@inmegen.gob.mx
|
| Phone |
+525553501909
|
| Organization name |
INMEGEN
|
| Department |
Research
|
| Lab |
UGAE Affymetrix
|
| Street address |
Periferico Sur 4124 Col. Ex rancho de Anzaldo
|
| City |
Mexico City |
| State/province |
DF |
| ZIP/Postal code |
01900 |
| Country |
Mexico |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM624008 |
MCF7 cell line _vehicle (control)_biological replicate 1 |
| GSM624009 |
MCF7 cell line _vehicle (control)_biological replicate 2 |
| GSM624010 |
MCF7 cell line _vehicle (control)_biological replicate 3 |
| GSM624011 |
MCF7 cell line_resveratrol_150mM_biological replicate 1 |
| GSM624012 |
MCF7 cell line_resveratrol_150mM_biological replicate 2 |
| GSM624013 |
MCF7 cell line_resveratrol_150mM_biological replicate 3 |
| GSM624014 |
MCF7 cell line_resveratrol_250mM_biological replicate 1 |
| GSM624015 |
MCF7 cell line_resveratrol_250mM_biological replicate 2 |
| GSM624016 |
MCF7 cell line_resveratrol_250mM_biological replicate 3 |
|
| Relations |
| BioProject |
PRJNA134157 |
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