|
| Status |
Public on Mar 19, 2024 |
| Title |
Identification of calcineurin-dependent, Crz1-independent gene expression changes in response to chronic CaCl2 stress |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Gene expression was quantified in S. cerevisae cells over a time course of three days of CaCl2 treatment to activate calcineurin. crz1 and crz1 cnb1 mutant strains were compared to allow the identification of calcineurin-dependent gene expression changes that are independent of the downstream transcription factor Crz1. We show that mitochondrial genes are downregulated in both geneotypes over time. Additionally, genes that promote glutathione biosynthesis are upregulated in cells that express calcineurin.
|
| |
|
| Overall design |
Gene expression was measured in cells lacking Crz1 (crz1) and cells lacking both Crz1 and calcineurin (crz1 cnb1). For each strain samples were collected before treatment and 12, 48 and 72 hours following the addition of 200mM CaCl2. Triplicate time course experiments were performed.
|
| |
|
| Contributor(s) |
Flynn MJ, Li R, Zhu LJ, Benanti JA |
| Citation(s) |
38562881 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 GM136280 |
Molecular Mechanisms of Cell Cycle Control |
UNIV OF MASSACHUSETTS MED SCHOOL |
Jennifer A Benanti |
|
| Submission date |
Jan 30, 2024 |
| Last update date |
Apr 17, 2024 |
| Contact name |
Jennifer Benanti |
| E-mail(s) |
Jennifer.Benanti@umassmed.edu
|
| Phone |
508-856-1773
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Molecular, Cell and Cancer Biology
|
| Street address |
364 Plantation St., LRB 525
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL25927 |
BGISEQ-500 (Saccharomyces cerevisiae) |
|
| Samples (24)
|
|
| This SubSeries is part of SuperSeries: |
| GSE254555 |
Identification of calcineurin-dependent gene expression changes in response to chronic stress |
|
| Relations |
| BioProject |
PRJNA1071031 |
Less...
More...