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Series GSE254552 Query DataSets for GSE254552
Status Public on Mar 19, 2024
Title Identification of calcineurin-dependent, Crz1-independent gene expression changes in response to chronic CaCl2 stress
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Summary Gene expression was quantified in S. cerevisae cells over a time course of three days of CaCl2 treatment to activate calcineurin. crz1 and crz1 cnb1 mutant strains were compared to allow the identification of calcineurin-dependent gene expression changes that are independent of the downstream transcription factor Crz1. We show that mitochondrial genes are downregulated in both geneotypes over time. Additionally, genes that promote glutathione biosynthesis are upregulated in cells that express calcineurin.
 
Overall design Gene expression was measured in cells lacking Crz1 (crz1) and cells lacking both Crz1 and calcineurin (crz1 cnb1). For each strain samples were collected before treatment and 12, 48 and 72 hours following the addition of 200mM CaCl2. Triplicate time course experiments were performed.
 
Contributor(s) Flynn MJ, Li R, Zhu LJ, Benanti JA
Citation(s) 38562881
NIH grant(s)
Grant ID Grant title Affiliation Name
R35 GM136280 Molecular Mechanisms of Cell Cycle Control UNIV OF MASSACHUSETTS MED SCHOOL Jennifer A Benanti
Submission date Jan 30, 2024
Last update date Apr 17, 2024
Contact name Jennifer Benanti
E-mail(s) Jennifer.Benanti@umassmed.edu
Phone 508-856-1773
Organization name University of Massachusetts Medical School
Department Molecular, Cell and Cancer Biology
Street address 364 Plantation St., LRB 525
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
 
Platforms (1)
GPL25927 BGISEQ-500 (Saccharomyces cerevisiae)
Samples (24)
GSM8045799 crz1_CaCl2_12_1
GSM8045800 crz1_CaCl2_12_2
GSM8045801 crz1_CaCl2_12_3
This SubSeries is part of SuperSeries:
GSE254555 Identification of calcineurin-dependent gene expression changes in response to chronic stress
Relations
BioProject PRJNA1071031

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE254552_crz_CaCl2_COUNT.csv.gz 347.7 Kb (ftp)(http) CSV
GSE254552_crz_CaCl2_TPM.csv.gz 945.4 Kb (ftp)(http) CSV
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Raw data are available in SRA

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