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| Status |
Public on Dec 25, 2010 |
| Title |
Gene expression analysis of embryonic photoreceptor precursor cells using BAC-Crx-EGFP transgenic mouse. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We generated a transgenic mouse line which express EGFP in the retina driven by the Crx promoter using BAC transgenesis. We sorted EGFP-positive photoreceptor precursors at E17.5 using FACS, and subsequently performed microarray analysis of the FACS-sorted cells.
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| Overall design |
In order to clarify a molecular role of Crx in developing cone photoreceptor precursors, we investigated the expression profile of the BAC-Crx-EGFP-positive cells compared with that of the BAC-Crx-EGFP-negative cells at E17.5.
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| |
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| Contributor(s) |
Muranishi Y, Furukawa T |
| Citation(s) |
20059961 |
| Submission date |
Nov 24, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Takahisa Furukawa |
| Organization name |
Osaka Bioscience Institute
|
| Department |
Developmental Biology
|
| Lab |
Furukawa Lab
|
| Street address |
6-2-4 Furuedai
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0874 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| GSM629314 |
Sorted positive cells at E17.5 rep1 |
| GSM629315 |
Sorted positive cells at E17.5 rep2 |
| GSM629316 |
Sorted negative cells at E17.5 rep1 |
| GSM629317 |
Sorted negative cells at E17.5 rep2 |
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| Relations |
| BioProject |
PRJNA134255 |
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