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| Status |
Public on Feb 05, 2011 |
| Title |
[E-MTAB-131] Estrogen Receptor alpha estrogen-responsive binding sites in MCF-7 |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We performed chromatin immunoprecipitation (ChIP) with two polyclonal anti-ERalpha antibodies directed against the N- and C-terminus of the protein, with or without estrogen treatment for 45 minutes of MCF-7 cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer.
ArrayExpress Release Date: 2009-07-27
Publication Title: Estrogen receptor alpha controls a gene network in luminal-like breast cancer cells comprising multiple transcription factors and microRNAs.
Publication Author List: Cicatiello L, Mutarelli M, Grober OM, Paris O, Ferraro L, Ravo M, Tarallo R, Luo S, Schroth GP, Seifert M, Zinser C, Chiusano ML, Traini A, De Bortoli M, Weisz A.
Person Roles: submitter
Person Last Name: Mutarelli
Person First Name: Margherita
Person Mid Initials:
Person Email: mutarelli@tigem.it
Person Phone:
Person Address:
Person Affiliation:
Person Roles: investigator
Person Last Name: Weisz
Person First Name: Alessandro
Person Mid Initials:
Person Email: alessandro.weisz@unina2.it
Person Phone:
Person Address:
Person Affiliation:
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| Overall design |
Experimental Design: compound_treatment_design
Experimental Design: in_vivo_design
Experimental Design: binding_site_identification_design
Experimental Design: high_throughput_sequencing_design
Experimental Design: ChIP-seq
Experimental Factor Name:COMPOUND
Experimental Factor Name:TIME
Experimental Factor Name:IMMUNOPRECIPITATE
Experimental Factor Type: compound
Experimental Factor Type: time
Experimental Factor Type: immunoprecipitate
Quality Control Type: real_time_PCR_quality_control
Quality Control Type: technical_replicate
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| Citation(s) |
20348243 |
| Submission date |
Nov 29, 2010 |
| Last update date |
Feb 21, 2019 |
| Organization |
European Bioinformatics Institute |
| E-mail(s) |
miamexpress@ebi.ac.uk
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| Lab |
ArrayExpress
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| Street address |
Wellcome Trust Genome Campus
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| City |
Hinxton |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB10 1SD |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (4)
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| Relations |
| ArrayExpress |
E-MTAB-131 |
| BioProject |
PRJNA135831 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE25684_RAW.tar |
367.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data provided as supplementary file |
| Raw data not provided for this record |
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