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| Status |
Public on Mar 02, 2024 |
| Title |
Impaired innate and adaptive immune responses to BNT162b2 SARSCoV-2 vaccination in systemic lupus erythematosus [CTD] |
| Platform organisms |
Escherichia coli; Triticum aestivum; Spodoptera frugiperda; Homo sapiens; Sus scrofa; Bos taurus; Capra hircus |
| Sample organism |
Homo sapiens |
| Experiment type |
Protein profiling by protein array
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| Summary |
We measued IgG autoantibodies associated with Connective Tissue Diseases (CTDs) and Anti-Cytokine Antibodies (ACA) in SLE and healthy controls who received the BNT162b2 vaccine.
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| Overall design |
We constructed protein arrays by first coupling recombinant protein antigens to uniquely fluorescent carboxylated magnetic beads. We then combined the beads into two seperate arrays that were used to probe patient SLE serum samples, healthy control samples, and autoimmune prototype samples. A Luminex FLEXMAP 3D® system was used to quantitatively measure autoantibody reactivity in each sample in units of mean flourescent intensity (MFI).
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| Contributor(s) |
Sarin KY, Zheng H, Chaichian Y, Arunachalam PS, Swaminathan G, Eschholz A, Gao F, Wirz O, Lam B, Yang E, Lee L, Feng A, Lewis MA, Lin J, Maecker HT, Boyd SD, Davis MM, Nadeau KC, Pulendran B, Khatri P, Utz PJ, Zaba LC |
| Citation missing |
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| Submission date |
Feb 28, 2024 |
| Last update date |
Mar 02, 2024 |
| Contact name |
Allan Feng |
| E-mail(s) |
afeng1@stanford.edu
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| Phone |
2055678956
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| Organization name |
Stanford School of Medicine
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| Department |
Imm/Rheum
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| Lab |
Utz
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| Street address |
269 Campus Dr
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
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| Samples (102)
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| Relations |
| BioProject |
PRJNA1081858 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE260478_CTD_Array_version_2_Platform_Raw_MFI_02272024.xlsx |
129.3 Kb |
(ftp)(http) |
XLSX |
| GSE260478_matrix_MFI.txt.gz |
14.7 Kb |
(ftp)(http) |
TXT |
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