|
| Status |
Public on Jan 03, 2011 |
| Title |
Widespread targeted chromatin remodeling during the initial phase of somatic cell reprogramming [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Despite rapid progress in characterizing transcription factor-driven reprogramming of somatic cells to an induced pluripotent stem (iPS) cell state, many mechanistic questions still remain. To gain insight into the earliest events in the reprogramming process, we systematically analyzed the transcriptional and epigenetic changes that occur during early factor induction after discrete numbers of divisions. We observed rapid, genome-wide changes in the euchromatic histone modification, H3K4me2, at more than a thousand loci including large subsets of pluripotency or developmentally related gene promoters and enhancers. In contrast, patterns of the repressive H3K27me3 modification remained largely unchanged except for focused depletion specifically at positions where H3K4 methylation is gained. These chromatin regulatory events precede transcriptional changes within the corresponding loci. Our data provide evidence for an early, organized, and population-wide epigenetic response to ectopic reprogramming factors that clarify the temporal order through which somatic identity is reset during reprogramming.
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| |
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| Overall design |
Covalent histone modifications were measured by ChIP-Seq during the initial phase in the reprogramming of mouse embryonic fibroblasts.
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| |
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| Contributor(s) |
Koche RP, Smith ZD, Meissner A |
| Citation(s) |
21211784 |
| Submission date |
Dec 16, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard Koche |
| Organization name |
Broad Institute of Harvard and MIT
|
| Street address |
185 Cambridge St.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
| Samples (22)
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| This SubSeries is part of SuperSeries: |
| GSE26100 |
Widespread targeted chromatin remodeling during the initial phase of somatic cell reprogramming |
|
| Relations |
| SRA |
SRP004904 |
| BioProject |
PRJNA142293 |
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