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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 01, 2011 |
| Title |
Mammalian Dpy-30 regulates genomic H3K4 methylation and is essential for ES cell fate specification |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
H3K4 methylation is associated with active genes and, along with H3K27 methylation, is part of a bivalent chromatin mark that typifies poised developmental genes in ESCs. However, its functional roles in ESC maintenance and differentiation are not established. Here we show that mammalian Dpy-30, a core subunit of SET1/MLL complexes, biochemically modulates H3K4 methylation in vitro, and directly regulates chromosomal H3K4me3 throughout the mammalian genome. Depletion of Dpy-30 does not affect ESC self-renewal, but significantly alters the differentiation potential of ESCs, particularly along the neural lineage. The differentiation defect is accompanied by defects in gene induction and H3K4 methylation at key developmental loci. Our results provide strong experimental evidence for the hypothesis that H3K4 methylation is an essential functional component of the bivalent mark during activation of developmental genes in ESCs.
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| Overall design |
Total RNAs from control or knockdown cells before and after RA-mediated differentiation were subjected to Illumina microarray analyses.
ChIP-enriched DNA from mouse ES cells was analyzed by Solexa sequencing.
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| Contributor(s) |
Jiang H, Roeder RG |
| Citation(s) |
21335234 |
| Submission date |
Dec 17, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Hao Jiang |
| E-mail(s) |
hjiang@rockefeller.edu
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| Organization name |
Rockefeller University
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| Lab |
Roeder Lab
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| Street address |
1230 York Ave
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (3) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (10)
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| Relations |
| SRA |
SRP006206 |
| BioProject |
PRJNA135385 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26136_RAW.tar |
1.3 Gb |
(http)(custom) |
TAR (of BED, TXT, WIG) |
| GSE26136_non-normalized.txt.gz |
2.2 Mb |
(ftp)(http) |
TXT |
| GSE26136_normalized.txt.gz |
2.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Processed data included within Sample table |
| Processed data are available on Series record |
| Raw data are available in SRA |
| Raw data are available on Series record |
| Processed data provided as supplementary file |
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