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| Status |
Public on May 01, 2024 |
| Title |
CUT&Tag Reveals a Return of Embryonic H3K4me3 Patterns During Zebrafish Adult Tail Fin Regeneration (CUT&Tag) |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Regenerative potential is governed by a complex process of transcriptional reprogramming, involving chromatin reorganization and dynamics in transcription factor binding patterns throughout the genome. The degree to which chromatin and epigenetic changes contribute to this process remains partially understood. Here we provide a modified CUT&Tag protocol suitable for improved characterization and interrogation of epigenetic changes during adult fin regeneration in zebrafish. Data generated from our protocol recapitulates results from previously published ChIP-Seq methods, requires far fewer cells as input, and significantly improves signal to noise ratios. We deliver high-resolution enrichment maps for H3K4me3 of uninjured and regenerating fin tissues. During regeneration, we find that H3K4me3 levels increase over gene promoters which become transcriptionally active and H3K4me3 is lost at genes which become silenced . Interestingly, these epigenetic reprogramming events recapitulate the H3K4me3 patterns observed in developing fin folds of 24 hour old zebrafish embryos. Our results indicate that changes in genomic H3K4me3 patterns during fin regeneration occur in a manner consistent with reactivation of developmental programs, demonstrating CUT&Tag to be an effective tool for profiling chromatin landscapes in regenerating tissues.
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| Overall design |
Cleavage Under Targets and Tagmentation sequencing and analysis for histone H3K4me3 in 24 hpf zebrafish fin fold, 0dpa, and 2dpa zebrafish adult caudal fins
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| Contributor(s) |
Phu D, Rodriguez-Parks A, Kang J, Murphy PJ |
| Citation(s) |
38645155 |
| Submission date |
Mar 13, 2024 |
| Last update date |
May 02, 2024 |
| Contact name |
Phu T Duong |
| E-mail(s) |
phu_duong@urmc.rochester.edu
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| Organization name |
University of Rochester
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| Street address |
1563 Elmwood Avenue, Apt 3
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| City |
Rochester, NY |
| State/province |
New York |
| ZIP/Postal code |
14620 |
| Country |
USA |
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| Platforms (1) |
| GPL24995 |
Illumina NovaSeq 6000 (Danio rerio) |
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| Samples (18)
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| GSM8146252 |
Adult Fin, H3K4me3, Cut&Tag, 0DPA, REP1 |
| GSM8146253 |
Adult Fin, H3K4me3, Cut&Tag, 0DPA, REP2 |
| GSM8146254 |
Adult Fin, H3K4me3, Cut&Tag, 0DPA, REP3 |
| GSM8146255 |
Adult Fin, H3K4me3, Cut&Tag, 2DPA, REP1 |
| GSM8146256 |
Adult Fin, H3K4me3, Cut&Tag, 2DPA, REP2 |
| GSM8146257 |
Adult Fin, H3K4me3, Cut&Tag, 2DPA, REP3 |
| GSM8146258 |
Fin Fold, H3K4me3, Cut&Tag, 24HPF, REP1 |
| GSM8146259 |
Fin Fold, H3K4me3, Cut&Tag, 24HPF, REP2 |
| GSM8146260 |
Fin Fold, H3K4me3, Cut&Tag, 24HPF, REP3 |
| GSM8146261 |
Adult Fin, IgG, Cut&Tag, 0DPA, REP1 |
| GSM8146262 |
Adult Fin, IgG, Cut&Tag, 0DPA, REP2 |
| GSM8146263 |
Adult Fin, IgG, Cut&Tag, 0DPA, REP3 |
| GSM8146264 |
Adult Fin, IgG, Cut&Tag, 2DPA, REP1 |
| GSM8146265 |
Adult Fin, IgG, Cut&Tag, 2DPA, REP2 |
| GSM8146266 |
Adult Fin, IgG, Cut&Tag, 2DPA, REP3 |
| GSM8146267 |
Fin Fold, IgG, Cut&Tag, 24HPF, REP1 |
| GSM8146268 |
Fin Fold, IgG, Cut&Tag, 24HPF, REP2 |
| GSM8146269 |
Fin Fold, IgG, Cut&Tag, 24HPF, REP3 |
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| This SubSeries is part of SuperSeries: |
| GSE261540 |
CUT&Tag Reveals a Return of Embryonic H3K4me3 Patterns During Zebrafish Tail Fin Regeneration |
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| Relations |
| BioProject |
PRJNA1087402 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE261538_RAW.tar |
150.0 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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