|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Mar 30, 2024 |
Title |
PEGboIFN-lambda as a FMD preexposure prophylactic and vaccine adjuvant in cattle—ISG and adaptive immunity gene expression |
Organism |
Bos taurus |
Experiment type |
Expression profiling by RT-PCR
|
Summary |
Foot-and-mouth disease (FMD) is a vesicular disease of cloven-hoofed animals with devastating economic implications. The current FMD vaccine, routinely used in enzootic countries, requires at least 7 days to induce protection. However, FMD vaccination is typically not recommended for use in non-enzootic areas, underscoring the need to develop new fast-acting therapies for FMD control during outbreaks. Interferons (IFNs) are among the immune system’s first line of defense against viral infections. Bovine type III IFN delivered by a replication defective adenovirus (Ad) vector has effectively blocked FMD in cattle. However, the limited duration of protection—usually only 1-3 days post-treatment (dpt)—diminishes its utility as a field therapeutic. Here we test whether polyethylene glycosylation (PEGylation) of recombinant bovine IFNλ3 (PEGboIFNλ3) can extend the duration of IFN-induced prevention of FMDV infection in both vaccinated and unvaccinated cattle. We treated groups of heifers with PEGboIFNλ3 alone or in combination with an adenovirus-based FMD O1Manisa vaccine (Adt-O1M) at either 3 or 5 days prior to challenge with homologous wild type FMDV. We found that pre-treatment with PEGboIFNλ3 was highly effective at preventing clinical FMD when administered at either time point, with or without co-administration of Adt-O1M vaccine. PEGboIFNλ3 protein was detectable systemically for >10 days and antiviral activity for 4 days following administration. Furthermore, in combination with Adt-O1M vaccine, we observed a strong induction of FMDV-specific IFNγ+ T cell response, demonstrating its adjuvanticity when co-administered with a vaccine. Our results demonstrate the promise of this modified IFN as a pre-exposure prophylactic therapy for use in emergency outbreak scenarios.
|
|
|
Overall design |
112 Samples, 3 biological replicates per treatment condition and time point and two technical replicates per sample.
|
|
|
Contributor(s) |
Attreed SE, Silva C, Rodriguez-Calzada M, Mogulothu A, Abbott S, Azzinaro P, Canning P, Skidmore L, Nelson J, Knudsen N, Medina GN, de los Santos T, Díaz-San Segundo F |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
Submission date |
Mar 21, 2024 |
Last update date |
Mar 30, 2024 |
Contact name |
Fayna Diaz San Segundo |
E-mail(s) |
fayna.diazsansegundo@nih.gov
|
Phone |
2409213931
|
Organization name |
NIH
|
Department |
NIAID-DMID-OBRRTR
|
Street address |
5601 Fisher Ln
|
City |
Rockville |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platforms (1) |
GPL34326 |
PIADC Mod5 Bovine ISG and Adaptive Immunity Gene RT-qPCR Panels |
|
Samples (112)
|
GSM8159303 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -5dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159304 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -5dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159305 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -5dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159306 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: -3dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159307 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: -3dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159308 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: -3dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159309 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: -3dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159310 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: -3dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159311 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: -3dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159312 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: -5dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159313 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: -5dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159314 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: -3dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159315 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: -3dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159316 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: -3dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159317 |
PBMC, Control treatment group, timepoint: -3dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159318 |
PBMC, Control treatment group, timepoint: -3dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159319 |
PBMC, Control treatment group, timepoint: -3dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159320 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -4dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159321 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -4dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159322 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -4dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159323 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: -2dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159324 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: -2dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159325 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: -2dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159326 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: -2dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159327 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: -2dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159328 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: -2dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159329 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: -4dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159330 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: -4dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159331 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: -2dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159332 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: -2dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159333 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: -2dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159334 |
PBMC, Control treatment group, timepoint: -2dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159335 |
PBMC, Control treatment group, timepoint: -2dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159336 |
PBMC, Control treatment group, timepoint: -2dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159337 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -3dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159338 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -3dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159339 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -3dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159340 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: -1dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159341 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: -1dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159342 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: -1dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159343 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: -1dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159344 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: -1dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159345 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: -1dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159346 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: -3dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159347 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: -3dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159348 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: -1dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159349 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: -1dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159350 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: -1dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159351 |
PBMC, Control treatment group, timepoint: -1dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159352 |
PBMC, Control treatment group, timepoint: -1dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159353 |
PBMC, Control treatment group, timepoint: -1dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159354 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -2dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159355 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -2dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159356 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -2dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159357 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: 0dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159358 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: 0dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159359 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: 0dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159360 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: 0dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159361 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: 0dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159362 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: 0dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159363 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: -2dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159364 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: -2dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159365 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -1dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159366 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -1dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159367 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: -1dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159368 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: -1dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159369 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: -1dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159370 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: 0dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159371 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: 0dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159372 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: 0dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159373 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: 0dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159374 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: 0dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159375 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: 14dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159376 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: 14dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159377 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: 14dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159378 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: 14dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159379 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: 14dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159380 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: 14dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159381 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: 14dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159382 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: 14dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159383 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: 14dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159384 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: 14dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159385 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: 14dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159386 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: 14dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159387 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: 14dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159388 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: 14dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159389 |
PBMC, Control treatment group, timepoint: 14dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159390 |
PBMC, Control treatment group, timepoint: 14dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159391 |
PBMC, Control treatment group, timepoint: 14dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159392 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: 21dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159393 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: 21dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159394 |
PBMC, PEGboIFNλ3+Ad-O1M(-5dpc) treatment group, timepoint: 21dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159395 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: 21dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159396 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: 21dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159397 |
PBMC, PEGboIFNλ3(-3dpc) treatment group, timepoint: 21dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159398 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: 21dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159399 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: 21dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159400 |
PBMC, PEGboIFNλ3+Ad-O1M(-3dpc) treatment group, timepoint: 21dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159401 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: 21dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159402 |
PBMC, PEGboIFNλ3(-5dpc) treatment group, timepoint: 21dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159403 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: 21dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159404 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: 21dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159405 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: 21dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159406 |
PBMC, Control treatment group, timepoint: 21dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159407 |
PBMC, Control treatment group, timepoint: 21dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159408 |
PBMC, Control treatment group, timepoint: 21dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159409 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: 0dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159410 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: 0dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159411 |
PBMC, Ad-O1M(-3dpc) treatment group, timepoint: 0dpc, biological replicate 3, technical replicate 1 and 2 |
GSM8159412 |
PBMC, Control treatment group, timepoint: 0dpc, biological replicate 1, technical replicate 1 and 2 |
GSM8159413 |
PBMC, Control treatment group, timepoint: 0dpc, biological replicate 2, technical replicate 1 and 2 |
GSM8159414 |
PBMC, Control treatment group, timepoint: 0dpc, biological replicate 3, technical replicate 1 and 2 |
|
Relations |
BioProject |
PRJNA1090536 |
Supplementary file |
Size |
Download |
File type/resource |
GSE262192_fold-change.txt.gz |
7.7 Kb |
(ftp)(http) |
TXT |
GSE262192_non-normalized.txt.gz |
9.6 Kb |
(ftp)(http) |
TXT |
|
|
|
|
|