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| Status |
Public on Apr 30, 2024 |
| Title |
EIF6 conditions drug-tolerant persister-like transdifferentiation in small cell lung carcinoma (eIF6_KD) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Drug-tolerant persister cells withstand treatments by adapting their identity through lineage-dependent plasticity during systemic anti-cancer therapies. This phenomenon is evident in small-cell lung carcinoma (SCLC), a lethal neuroendocrine cancer initially responsive (60-80%) to platinum-based chemotherapy but succumbing to resistance within 6 months in advanced stages. This resistance associates with the transdifferentiation of residual tumour cells into a non-neuroendocrine state, a process intricately tied to SCLC's chemotolerance, yet molecular mechanisms governing this lineage conversion remain completed understood. Here we use paired cytoplasmic RNA-seq and polysomal RNA-seq to compare gene expression between NE and non-NE SCLC cell lines on both transcriptional and translational level. We report that first-line chemotherapy induces translation initiation factor eIF6 in drug-tolerant persister-like cells in SCLC, correlating with the non-neuroendocrine state in SCLC. Intervening eIF6 inhibits non-neuroendocrine transdifferentiation, thus enhancing SCLC responsiveness to chemotherapy. This study sheds light on eIF6's potential therapeutic interventions to mitigate treatment resistance in SCLC.
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| Overall design |
To investigate function of eIF6 in H69 and H69M cells, we established stable eIF6-KD H69 and H69M cell lines by lenitivirus infection containing shRNA targeting eIF6.
Comparative gene expression profiling analysis of RNA-seq data for H69_sheIF6 cells vs. H69_shNTC cells and H69M_sheIF6 cells vs. H69M_shNTC cells
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| Contributor(s) |
Peng H, Wang Z, Wang M, Liu L, Shen S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 27, 2024 |
| Last update date |
Apr 30, 2024 |
| Contact name |
Haoning Peng |
| Organization name |
West China Hospital, Sichuan University
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| Department |
Department of Thoracic Surgery
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| Lab |
Institute of Thoracic Oncology
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| Street address |
No.37 Guoxue Alley, Wuhou District
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| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610041 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (16)
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| GSM8172173 |
H69 cells, shNTC, cytoplasmic RNA, replicate 1 |
| GSM8172174 |
H69 cells, shNTC, cytoplasmic RNA, replicate 2 |
| GSM8172175 |
H69 cells, sheIF6, cytoplasmic RNA, replicate 1 |
| GSM8172176 |
H69 cells, sheIF6, cytoplasmic RNA, replicate 2 |
| GSM8172177 |
H69 cells, shNTC, polysomal RNA, replicate 1 |
| GSM8172178 |
H69 cells, shNTC, polysomal RNA, replicate 2 |
| GSM8172179 |
H69 cells, sheIF6, polysomal RNA, replicate 1 |
| GSM8172180 |
H69 cells, sheIF6, polysomal RNA, replicate 2 |
| GSM8172181 |
H69M cells, shNTC, cytoplasmic RNA, replicate 1 |
| GSM8172182 |
H69M cells, shNTC, cytoplasmic RNA, replicate 2 |
| GSM8172183 |
H69M cells, sheIF6, cytoplasmic RNA, replicate 1 |
| GSM8172184 |
H69M cells, sheIF6, cytoplasmic RNA, replicate 2 |
| GSM8172185 |
H69M cells, shNTC, polysomal RNA, replicate 1 |
| GSM8172186 |
H69M cells, shNTC, polysomal RNA, replicate 2 |
| GSM8172187 |
H69M cells, sheIF6, polysomal RNA, replicate 1 |
| GSM8172188 |
H69M cells, sheIF6, polysomal RNA, replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE262597 |
EIF6 conditions drug-tolerant persister-like transdifferentiation in small cell lung carcinoma |
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| Relations |
| BioProject |
PRJNA1092618 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE262596_eIF6_KD_count.txt.gz |
911.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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