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| Status |
Public on Apr 15, 2024 |
| Title |
Transcriptional profile of human monocyte-derived macrophages stimulated with Interleukin 27 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Interleukin 27 (IL-27) is a member of the IL-6 and/or IL-12 family that elicits both pro- and anti-inflammatory responses. Interferons (IFNs) are a family of cytokines that activate the JAK-STAT signaling pathway to induce an antiviral state in cells. Recent studies have reported that IL-27 also induces a robust antiviral response against diverse viruses, both in vitro and in vivo, suggest that IFNs and IL-27 share many similarities at the functional level. However, it is still unknown how similar or different IFN- and IL-27-dependent signaling pathways are. We reported that IFNs and IL-27 activate JAK-STAT signaling and induce shared pro-inflammatory and antiviral pathways in monocyte-derived macrophages (MDMs), leading to the common and unique expression of inflammatory factors and IFN-stimulated genes (ISGs). Importantly, the ability of IL-27 to induce those responses is independent of IFN induction and cellular lineage. Additionally, functional analysis demonstrated that like IFNs, IL-27-mediated response reduced chikungunya and dengue viruses replication in MDMs. In summary, IL-27 exhibits properties similar to those of all three types of human IFN, including the ability to stimulate a protective antiviral response.
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| Overall design |
Human primary FBS-MDMs cultures (n= 3) were either stimulated or not with 25 ng/mL recombinant-human IL27 for 18 hours and mRNA sequencing was then performed using bulk RNA-seq
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| Citation missing |
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| Submission date |
Apr 01, 2024 |
| Last update date |
Apr 15, 2024 |
| Contact name |
Yordi Sebastián Tamayo Molina |
| E-mail(s) |
ystm0827@gmail.com
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| Organization name |
University of Antioquia
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| Lab |
Inmunovirology
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| Street address |
Street 67 No. 53 - 108
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| City |
Medellin |
| State/province |
Antioquia |
| ZIP/Postal code |
050010 |
| Country |
Colombia |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1095003 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE262963_raw_counts.txt.gz |
725.5 Kb |
(ftp)(http) |
TXT |
| GSE262963_raw_counts_batch_corrected.txt.gz |
379.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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