|
| Status |
Public on May 01, 2024 |
| Title |
Tracking live-cell single-molecule dynamics enables measurements of heterochromatin-associated protein-protein interactions |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Visualizing and measuring molecular-scale interactions in living cells represents a major challenge, but recent advances in microscopy are bringing us closer to achieving this goal. Single-molecule super-resolution microscopy enables high-resolution and sensitive imaging of the positions and movement of molecules in living cells. HP1 proteins are important regulators of gene expression because they selectively bind and recognize H3K9 methylated (H3K9me) histones to form heterochromatin-associated protein complexes that silence gene expression. Here, we extended live-cell single-molecule tracking studies in fission yeast to determine how HP1 proteins interact with their binding partners in the nucleus. We measured how genetic perturbations that affect H3K9me alter the diffusive properties of HP1 proteins and each of their binding partners based on which we inferred their most likely interaction sites. Our results indicate that H3K9me promotes specific complex formation between HP1 proteins and their interactors in a spatially restricted manner, while attenuating their ability to form off-chromatin complexes. As opposed to being an inert platform or scaffold to direct HP1 binding, our studies propose a novel function for H3K9me as an active participant in enhancing HP1-associated complex formation in living cells.
|
| |
|
| Overall design |
Chromatin immunoprecipitation seqeuencing (ChIP-Seq) for Chp2 tagged with PAmCherry using a mCherry antibody
|
| |
|
| Contributor(s) |
Chen Z, Seman M, Farhat A, Fyodorova Y, Biswas S, Levashkevich A, Freddolino PL, Biteen JS, Ragunathan K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 08, 2024 |
| Last update date |
May 01, 2024 |
| Contact name |
Melissa Seman |
| E-mail(s) |
mseman@brandeis.edu
|
| Organization name |
Brandeis University
|
| Street address |
415 South St
|
| City |
Waltham |
| ZIP/Postal code |
02453 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20584 |
Illumina NextSeq 500 (Schizosaccharomyces pombe) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA1097604 |
Less...
More...