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| Status |
Public on May 13, 2024 |
| Title |
Selective CAR-T cell mediated B cell depletion suppresses interferon signature in SLE [CARTProject_Bcells] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Applying advanced molecular profiling together with highly specific targeted therapies offers the possibility to better dissect the mechanisms underlying immune mediated inflammatory diseases such as systemic lupus erythematosus (SLE) in humans. Here we apply a combination of single cell RNA sequencing and T/B cell repertoire analysis to perform an in-depth characterization of molecular changes in the immune-signature upon CD19 CAR T cell-mediated depletion of B cells in SLE patients. The resulting datasets do not only confirm a selective CAR T cell-mediated reset of the B cell response, but simultaneously reveal consequent changes in the transcriptional signature of monocyte and T cell subsets that respond with a profound reduction in type 1 interferon signaling. Our current data thus provide evidence for a causal relationship between the B cell response and the increased interferon signature observed in SLE and additionally demonstrate the usefulness of combining targeted therapies and novel analytic approaches to decipher molecular mechanisms of immune-mediated inflammatory diseases in humans.
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| Overall design |
We generated single cell sequencing data of isolated B cells from SLE patients prior to and after anti CD19 CAR-T cell therapy
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| Contributor(s) |
Wilhelm A, Chambers D, Müller F, Bozec A, Grieshaber-Bouyer R, Winkler T, Mougiakakos D, Mackensen A, Schett G, Krönke G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 15, 2024 |
| Last update date |
May 14, 2024 |
| Contact name |
Artur Wilhelm |
| E-mail(s) |
Artur.wilhelm@charite.de
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| Organization name |
Charite Berlin
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| Department |
Rheumatology and clinical immunology
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| Lab |
AG Krönke
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| Street address |
Virchowweg 11
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| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (20)
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| GSM8207627 |
Patient1, pre CART-cell Treatment, scRNAseq |
| GSM8207628 |
Patient1, post CART-cell Treatment, scRNAseq |
| GSM8207629 |
Patient3, pre CART-cell Treatment, scRNAseq |
| GSM8207630 |
Patient3, post CART-cell Treatment, scRNAseq |
| GSM8207631 |
Patient5, pre CART-cell Treatment, scRNAseq |
| GSM8207632 |
Patient5, post CART-cell Treatment, scRNAseq |
| GSM8207633 |
Patient6, pre CART-cell Treatment, scRNAseq |
| GSM8207634 |
Patient6, post CART-cell Treatment, scRNAseq |
| GSM8207635 |
Patient7, pre CART-cell Treatment, scRNAseq |
| GSM8207636 |
Patient7, post CART-cell Treatment, scRNAseq |
| GSM8207637 |
Patient1, pre CART-cell Treatment, BCRseq |
| GSM8207638 |
Patient1, post CART-cell Treatment, BCRseq |
| GSM8207639 |
Patient3, pre CART-cell Treatment, BCRseq |
| GSM8207640 |
Patient3, post CART-cell Treatment, BCRseq |
| GSM8207641 |
Patient5, pre CART-cell Treatment, BCRseq |
| GSM8207642 |
Patient5, post CART-cell Treatment, BCRseq |
| GSM8207643 |
Patient6, pre CART-cell Treatment, BCRseq |
| GSM8207644 |
Patient6, post CART-cell Treatment, BCRseq |
| GSM8207645 |
Patient7, pre CART-cell Treatment, BCRseq |
| GSM8207646 |
Patient7, post CART-cell Treatment, BCRseq |
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| Relations |
| BioProject |
PRJNA1100403 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE263932_RAW.tar |
366.9 Mb |
(http)(custom) |
TAR (of CSV, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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