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| Status |
Public on Feb 09, 2011 |
| Title |
Genome-wide binding of STAT3 and STAT5 under Th17 conditions (ChIP-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Interleukin 2 (IL-2), a cytokine linked to human autoimmune diseases, limits IL-17 production. We show that deletion of Stat3 in T cells abrogates IL-17 production and attenuates autoimmunity associated with IL-2 deficiency. While STAT3 induces IL-17 and RORγt and inhibits Foxp3, IL-2 inhibited IL-17 independently of Foxp3 and RORγt. We found that STAT3 and STAT5 bound to multiple common sites across the Il17 genetic locus. The induction of STAT5 binding by IL-2 was associated with a reduction in STAT3 binding at these sites and the inhibition of associated active epigenetic marks. Titrating the relative activation of STAT3 and STAT5 modulated TH17 cell specification. Thus, the balance rather than the absolute magnitude of these signals determines the propensity of cells to make a key inflammatory cytokine.
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| Overall design |
The genome-wide binding of STAT3 and STAT5 under Th17 conditions was investigated by CHIP-seq.
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| Contributor(s) |
Yang X, Ghoreschi K, Steward-Tharp SM, Rodriguez-Canales J, Zhu J, Grainger JR, Hirahara K, Sun H, Wei L, Vahedi G, Kanno Y, O’Shea JJ, Laurence A |
| Citation(s) |
21278738 |
| Submission date |
Jan 11, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Golnaz Vahedi |
| Organization name |
National Institutes of Health
|
| Department |
NIAMS
|
| Lab |
Lymphocyte Cell Biology Section
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| Street address |
9000 Rockville Pike Bldg 10 Rm 13C101A
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE26553 |
Opposing regulation of the Il17 locus through direct, reciprocal actions of STAT3 and STAT5 |
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| Relations |
| SRA |
SRP005277 |
| BioProject |
PRJNA142287 |
