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| Status |
Public on Apr 29, 2024 |
| Title |
lncRNA profile of CAFs derived EVs |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Perineural invasion (PNI) is a unique biological feature of pancreatic cancer and is a key cause of pancreatic cancer metastasis, recurrence and poor postoperative survival, but its mechanism is largely unclarified. Clinical sample analysis and endoscopic ultrasonographic elasticity scoring indicated that cancer-associated fibroblasts (CAFs) are closely related to the occurrence of PNI. Furthermore, CAF-derived extracellular vesicles played an extremely important role in PNI in a dorsal root ganglion (DRG) coculture model and sciatic nerve model. To explore the lncRNA packaged in CAFs EVs, we conducted the lncRNA profile of CAFs derived EVs.
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| Overall design |
NFs and CAFs were isolated from pancreatic cancer and conditional medium were collected for EVs isolation. Then the RNA packaged by EVs were extracted for lncRNA-seq.
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| Contributor(s) |
Zheng S, Hu C, Ting T, Chen R |
| Citation missing |
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| Submission date |
Apr 24, 2024 |
| Last update date |
Apr 29, 2024 |
| Contact name |
li xiaobai |
| E-mail(s) |
litingting1833@gmail.com
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| Organization name |
south china university of technology
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| Street address |
381 Wushan Road, Tianhe District, Guangzhou
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| City |
广州 |
| ZIP/Postal code |
510641 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1104171 |
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