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| Status |
Public on May 13, 2024 |
| Title |
Regulation of Microglia-Mediated Neuroinflammation Post-Intracerebral Hemorrhage by Gut Microbiota and Short-Chain Fatty Acid Metabolism |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Intracerebral hemorrhage (ICH) induces alterations in the gut microbiota composition, significantly impacting neuroinflammation post-ICH. However, the impact of gut microbiota absence on neuroinflammation following ICH-induced brain injury remain unexplored. Here, we observed that the gut microbiota absence was associated with reduced neuroinflammation, alleviated neurological dysfunction, and mitigated gut barrier dysfunction post-ICH. In contrast, recolonization of microbiota from ICH-induced SPF mice by transplantation of fecal microbiota (FMT) exacerbated brain injury and gut impairment post-ICH. Additionally, microglia with transcriptional changes mediated the protective effects of gut microbiota absence on brain injury, with Apoe emerging as a hub gene. Subsequently, Apoe deficiency in peri-hematomal microglia was associated with improved brain injury. Finally, we revealed that gut microbiota influence brain injury and gut impairment via gut-derived short-chain fatty acids (SCFA).
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| Overall design |
To investigate the complex modulatory effects of the gut microbiota on microglia following intracerebral hemorrhage, we established a intracerebral hemorrhage collagenase model and corresponding sham surgery controls using antibiotic cocktail-treated ABX mice, germ-free mice, and SPF mice. Subsequently, on days 3 and 7 post-cerebral hemorrhage, we performed RNA sequencing on the microglia surrounding the hematoma using flow cytometry-based cell sorting, and analyzed the RNA-Seq data to obtain gene expression profiles.
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| Contributor(s) |
He X, Fu X, Li Y, Wang L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 05, 2024 |
| Last update date |
May 13, 2024 |
| Contact name |
Xuchao He |
| E-mail(s) |
xuchaohe@zju.edu.cn
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| Phone |
19857131935
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| Organization name |
zhejiang university
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| Street address |
88 jiefang RD
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| City |
hangzhou |
| State/province |
zhejiang |
| ZIP/Postal code |
310009 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (27)
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| GSM8252309 |
microglia,ABX_ICH,Day7_1 |
| GSM8252310 |
microglia,ABX_ICH,Day7_2 |
| GSM8252311 |
microglia,ABX_ICH,Day7_3 |
| GSM8252312 |
microglia,ABX_Sham,Day7_1 |
| GSM8252313 |
microglia,ABX_Sham,Day7_2 |
| GSM8252314 |
microglia,ABX_Sham,Day7_3 |
| GSM8252315 |
microglia,GF_ICH,Day3_1 |
| GSM8252316 |
microglia,GF_ICH,Day3_2 |
| GSM8252317 |
microglia,GF_ICH,Day3_3 |
| GSM8252318 |
microglia,GF_ICH,Day7_1 |
| GSM8252319 |
microglia,GF_ICH,Day7_2 |
| GSM8252320 |
microglia,GF_ICH,Day7_3 |
| GSM8252321 |
microglia,GF_Sham,Day7_1 |
| GSM8252322 |
microglia,GF_Sham,Day7_2 |
| GSM8252323 |
microglia,GF_Sham,Day7_3 |
| GSM8252324 |
microglia,SPF_ICH,Day3_1 |
| GSM8252325 |
microglia,SPF_ICH,Day3_2 |
| GSM8252326 |
microglia,SPF_ICH,Day3_3 |
| GSM8252327 |
microglia,SPF_ICH,Day7_1 |
| GSM8252328 |
microglia,SPF_ICH,Day7_2 |
| GSM8252329 |
microglia,SPF_ICH,Day7_3 |
| GSM8252330 |
microglia,SPF_Sham,Day7_1 |
| GSM8252331 |
microglia,SPF_Sham,Day7_2 |
| GSM8252332 |
microglia,SPF_Sham,Day7_3 |
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| Relations |
| BioProject |
PRJNA1107924 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE266602_gene_count_matrix.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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