 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 22, 2024 |
| Title |
Single-cell multiomics profiling reveals heterogeneous transcriptional programs and microenvironment in Desmoplastic Small Round Cell Tumors [ATAC-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Desmoplastic Small Round Cell Tumor (DSRCT) is a rare, aggressive sarcoma driven by the EWSR1-WT1 chimeric transcription factor. Despite this unique oncogenic driver, DSRCT displays a polyphenotypic differentiation of unknown causality. Using single-cell multi-omics on samples from patients, we find that DSRCT tumor cells cluster into consistant subpopulations with partially overlapping lineage- and metabolism-related transcriptional programs.
|
| |
|
| Overall design |
ATAC-seq was performed in the JN-DSRCT-1 cell line after a 48-hour siRNA-mediated silencing using a control non-targeting siRNA, a CCND1-targeting siRNA, a EWSR1::WT1 siRNA, or in a control condition without transfection.
|
| |
|
| Contributor(s) |
Henon C, Vibert J, Colmet-Daage L, Postel-Vinay S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 18, 2024 |
| Last update date |
May 22, 2024 |
| Contact name |
Clémence Henon |
| E-mail(s) |
clemence.henon@gustaveroussy.fr
|
| Organization name |
Gustave Roussy
|
| Street address |
114 rue Edouard Vaillant
|
| City |
Villejuif |
| ZIP/Postal code |
94800 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (8)
|
| GSM8279116 |
JN-DSRCT-1, siCTRL, R2, ATAC |
| GSM8279117 |
JN-DSRCT-1, siCCND1, R1, ATAC |
| GSM8279118 |
JN-DSRCT-1, siCCND1, R2, ATAC |
| GSM8279119 |
JN-DSRCT-1, siEWSR1::WT1, R1, ATAC |
| GSM8279120 |
JN-DSRCT-1, siEWSR1::WT1, R2, ATAC |
|
| This SubSeries is part of SuperSeries: |
| GSE263523 |
Single-cell multiomics profiling reveals heterogeneous transcriptional programs and microenvironment in Desmoplastic Small Round Cell Tumors |
|
| Relations |
| BioProject |
PRJNA1113159 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE267807_RAW.tar |
7.5 Gb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...