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| Status |
Public on Sep 13, 2013 |
| Title |
Optimized CD8 effector TL |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptome analyses of naive, effector and memory CD8 TCRP1A lymphocytes expressing or not an active form of the transcription factor Stat5.
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| Overall design |
TCRP1A CD8 T lymphocytes were activated by their cognate Ag for 72h to induce their differentiation in effector T cells (TCRP1A eTL 72h: 4 replicates S1, S2, S3, S4). In some samples, an active form of Stat5 was introduced (TCRP1A Stat5ca eTL 72h: 2 replicates S9, S10). These 72h activated T cells were either purified and analyzed directly (samples mentioned above) or injected in congeneic hosts and recovered more than 20 days later from the host spleen and lymph nodes: TCRP1A eTL >d20: 2 replicates– S30, S32; TCRP1A Stat5ca eTL >d20: 4 replicates S11, S12, S13, S14). Naive TCRP1A CD8 T lymphocytes (TCRP1A-naive: 4 replicates S33, S34, S35, S36) are included as controls.
TCRP1A CD8 T lymphocytes were activated by anti-CD3/CD28. After 24h, an active form of Stat5 was introduced in activated cells. Culture was continued for another 48h to induce their differentiation in effector T cells. These 72h activated T cells were either directly injected in congeneic hosts and recovered more than 14 days later from the host spleen and lymph nodes: T-BetKO Stat5ca >d14: 3 replicates S39, S40, S41.
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| Contributor(s) |
Auphan-Anezin N, Schmitt-Verhulst A, Barbry P, Lebrigand K |
| Citation(s) |
24006458 |
| Submission date |
Jan 28, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Nathalie AUPHAN-ANEZIN |
| E-mail(s) |
auphan@ciml.univ-mrs.fr
|
| Phone |
+33 491 269 189
|
| Organization name |
CIML
|
| Street address |
Parc Scientifique de Luminy
|
| City |
MARSEILLE |
| ZIP/Postal code |
13009 |
| Country |
France |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (19)
|
| GSM663434 |
activated (72h) CD8 T lymphocytes transduced to express Stat5ca S9 |
| GSM663435 |
naive peripheral CD8 T lymphocytes S34 |
| GSM663436 |
activated (72h) CD8 T lymphocytes S4 |
| GSM663437 |
activated (72h) CD8 T lymphocytes S3 |
| GSM663438 |
activated (72h) CD8 T lymphocytes S2 |
| GSM663439 |
activated CD8 T lymphocytes transduced to express Stat5ca and transferred in congenic hosts (d20) S14 |
| GSM663440 |
activated (72h) CD8 T lymphocytes S1 |
| GSM663441 |
naive peripheral CD8 T lymphocytes S33 |
| GSM663442 |
activated CD8 T lymphocytes transferred in congenic hosts (d120) S32 |
| GSM663443 |
activated CD8 T lymphocytes transferred in congenic hosts (d18) S30 |
| GSM663444 |
activated CD8 T lymphocytes transduced to express Stat5ca and transferred in congenic hosts (d20) S13 |
| GSM663445 |
activated CD8 T lymphocytes transduced to express Stat5ca and transferred in congenic hosts (d20) S12 |
| GSM663446 |
activated CD8 T lymphocytes transduced to express Stat5ca and transferred in congenic hosts (d20) S11 |
| GSM663447 |
naive peripheral CD8 T lymphocytes S36 |
| GSM663448 |
activated (72h) CD8 T lymphocytes transduced to express Stat5ca S10 |
| GSM663449 |
naive peripheral CD8 T lymphocytes S35 |
| GSM1024796 |
S41-T-BetKO Stat5ca d54 |
| GSM1024797 |
S40-T-BetKO Stat5ca d14 |
| GSM1024798 |
S39-T-BetKO Stat5ca d14 |
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA136945 |
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