|
| Status |
Public on Jan 29, 2011 |
| Title |
Programming of marginal zone B cell fate by basic krüppel-like factor (BKLF/KLF3) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression analysis of splenic follicular B cells and marginal zone B cells from B6 and CD19:KLF3 transgenic mice
Comparing KLF3-transgenic and non-transgenic follicular B cells by RNA-microarray revealed that KLF3 regulates a subset of genes that was similarly up-/downregulated upon normal MZ B cell differentiation. Indeed, KLF3 expression overcame the lack of MZ B cells caused by different genetic alterations, such as CD19-deficiency or blockade of B-cell activating factor (BAFF)-receptor signaling, indicating that KLF3 may complement alternative NF-κB signaling. Thus, KLF3 is a driving force towards MZ B cell maturation.
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| |
|
| Overall design |
RNA of splenic follicular B cells and marginal zone B cells were obtained from 4 different mice per group (B6 and CD19:KLF3 mice). 16 samples = 8 individual mice x 2 B cell subsets.
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| |
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| Contributor(s) |
Agenès F, Goulet J, Loubert J, Kirberg J |
| Citation(s) |
21297003 |
| Submission date |
Jan 28, 2011 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Jean-Philippe Goulet |
| E-mail(s) |
jp.goulet@umontreal.ca
|
| Organization name |
Université de Montréal
|
| Street address |
C.P. 6128, succursale Centre-ville
|
| City |
Montréal |
| State/province |
Québec |
| ZIP/Postal code |
H3X 3J7 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (16)
|
| GSM663460 |
Spleen MZB cells from B6 mouse #92758 |
| GSM663461 |
Spleen Follicular B cells from B6 mouse #92758 |
| GSM663462 |
Spleen MZB cells from CD19:KLF3 mouse #92759 |
| GSM663463 |
Spleen Follicular B cells from CD19:KLF3 mouse #92759 |
| GSM663464 |
Spleen MZB cells from CD19:KLF3 mouse #92747 |
| GSM663465 |
Spleen Follicular B cells from CD19:KLF3 mouse #92747 |
| GSM663466 |
Spleen MZB cells from B6 mouse #92748 |
| GSM663467 |
Spleen Follicular B cells from B6 mouse #92748 |
| GSM663468 |
Spleen MZB cells from B6 mouse #92750 |
| GSM663469 |
Spleen Follicular B cells from B6 mouse #92750 |
| GSM663470 |
Spleen MZB cells from CD19:KLF3 mouse #92751 |
| GSM663471 |
Spleen Follicular B cells from CD19:KLF3 mouse #92751 |
| GSM663472 |
Spleen MZB cells from CD19:KLF3 mouse #92768 |
| GSM663473 |
Spleen Follicular B cells from CD19:KLF3 mouse #92768 |
| GSM663474 |
Spleen MZB cells from B6 mouse #92769 |
| GSM663475 |
Spleen Follicular B cells from B6 mouse #92769 |
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| Relations |
| BioProject |
PRJNA136951 |
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