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| Status |
Public on Feb 28, 2011 |
| Title |
Comparison of gene expression data between control and tissue specific epicardial PDGF receptor (a and b) knockout embryonic hearts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Epicardial cells undergo an epithelial-to-mesenchymal transtion (EMT) to generate coronary vascular smooth muscle cells (VSMC) and cardiac fibroblasts. Little is known about the mechanisms regulating EMT or the in vivo signals directing epicardial-derived cell (EPDC) fate. Here, we show that loss of PDGF signaling leads to a disruption in Sox9 expression, and when Sox9 expression was restored in mutant hearts, the EMT defect was rescued. Interestingly, mutants lacking only one of the PDGF genes exhibited a lineage specific requirement for the individual receptors. Loss of PDGFRα resulted in a deficit in cardiac fibroblast formation, while cVSMC development was unperturbed. Conversely, PDGFRβ was required for cVSMC development but not cardiac fibroblast development. Combined, our data demonstrate a novel role for PDGF receptors in epicardial EMT and EPDC development.
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| Overall design |
GSM671723-GSM671724: Total RNA was isolated from E12.5 control and PDGF receptor epicardial knockout hearts using the Trizol reagent. RNA was processed as per manufacturer's instructions (Illumina Gene expression array, Illumina, inc. San Diego, CA, USA)
GSM671877-GSM671882: Total RNA was isolated from E12.5 control and PDGF receptor epicardial knockout primary epicardial cultures using the Trizol reagent. RNA was processed as per manufacturer's instructions (Illumina Gene expression array, Illumina, inc. San Diego, CA, USA)
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| Contributor(s) |
Tallquist M, Smith C |
| Citation(s) |
21512159 |
| Submission date |
Feb 09, 2011 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Michelle Tallquist |
| E-mail(s) |
Michelle.Tallquist@utsouthwestern.edu, Christopher.Smith@utsouthwestern.edu
|
| Phone |
214-648-5180
|
| URL |
http://www.utsouthwestern.edu/tallquistlab/
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Department |
Molecular Biology
|
| Lab |
Tallquist Lab
|
| Street address |
5323 Harry Hines BLVD MC9148
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (8)
|
| GSM671878 |
E12.5 PDGFR (a and b) epicardial tissue specific knockout culture |
| GSM671879 |
E12.5 wild type PDGFBB treated culture - 12h |
| GSM671880 |
E12.5 wild type PDGFBB treated culture - 24h |
| GSM671881 |
E12.5 wild type Vehicle treated culture |
| GSM671882 |
E12.5 wild type PDGFBB, bFGF, TGFb1 treated culture |
|
| Relations |
| BioProject |
PRJNA137373 |
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