|
| Status |
Public on Jun 01, 2011 |
| Title |
FZD7 Plays a Critical Role in Triple Negative Breast Cancer Proliferation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Breast cancer is genetically and clinically heterogeneous. Triple negative cancer (TNBC) is a subtype of breast cancer usually associated with poor outcome and lack of benefit from target therapy. A pathway analysis in a microarray study was performed using TNBC compared with non-triple negative breast cancer (non-TNBC). Overexpression of several Wnt pathway genes, such as frizzled homolog 7 (FZD7), Low density lipoprotein receptor-related protein 6 (LRP6) and transcription factor 7 (TCF7) has been observed in TNBC. Focus was given to the Wnt pathway receptor, FZD7. To validate its function, inhibition of FZD7 using FZD7shRNA was carried out. Notably decreased cell proliferation, suppressed invasiveness and colony formation in triple negative MDA-MB-231 and BT-20 cells were observed. Mechanism study indicated that these effects occurred through silencing the canonical Wnt signaling pathway, as evidenced by loss of nuclear accumulation of beta-catenin and decreased transcriptional activity of TCF7. In vivo study revealed that FZD7shRNA significantly suppressed the tumor formation in xenotransplation mice due to decrease cell proliferation. Our finding suggests that FZD7 involved canonical Wnt signaling pathway is essential for tumorigenesis of TNBC. Thus, FZD7 may be a biomarker and a potential therapeutic target for triple negative breast cancer.
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| |
|
| Overall design |
14 pretreatment non-triple negative breast tumors compare with 5 triple negative breast tumor.
|
| |
|
| Contributor(s) |
Yang L, Wu X, Yen Y |
| Citation(s) |
21532620 |
| Submission date |
Feb 22, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Xiwei Wu |
| E-mail(s) |
xwu@coh.org
|
| Organization name |
City of Hope National Medical Center
|
| Department |
Computational and Quantitative Medicine
|
| Street address |
1500 E. Duarte Rd.
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (19)
|
| GSM678364 |
non-triple negative breast cancer tumor B2 |
| GSM678365 |
non-triple negative breast cancer tumor B4 |
| GSM678366 |
non-triple negative breast cancer tumor B5 |
| GSM678367 |
non-triple negative breast cancer tumor B8 |
| GSM678368 |
non-triple negative breast cancer tumor B9 |
| GSM678369 |
triple negative breast cancer tumor B12 |
| GSM678370 |
non-triple negative breast cancer tumor B14 |
| GSM678371 |
non-triple negative breast cancer tumor B17 |
| GSM678372 |
non-triple negative breast cancer tumor B20 |
| GSM678373 |
triple negative breast cancer tumor B23 |
| GSM678374 |
non-triple negative breast cancer tumor B24 |
| GSM678375 |
triple negative breast cancer tumor B26 |
| GSM678376 |
non-triple negative breast cancer tumor B27 |
| GSM678377 |
non-triple negative breast cancer tumor B30 |
| GSM678378 |
triple negative breast cancer tumor B31 |
| GSM678379 |
non-triple negative breast cancer tumor B32 |
| GSM678380 |
non-triple negative breast cancer tumor B33 |
| GSM678381 |
non-triple negative breast cancer tumor B34 |
| GSM678382 |
triple negative breast cancer tumor B99 |
|
| Relations |
| BioProject |
PRJNA137075 |
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