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| Status |
Public on Aug 03, 2011 |
| Title |
MiR-192 inhibits nucleotide excision repair by targeting ERCC3 and ERCC4 in HepG2.2.15 cells. |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Deficient DNA repair capacity is associated with genetic lesions accumulation and susceptibility to carcinogenesis. MicroRNAs (miRNAs) are small non-coding RNAs that regulate various cellular pathways including DNA repair. Here we hypothesized that the existence of HBV products may interfere with cellular nucleotide excision repair (NER) through microRNA-mediated gene regulation. We found that NER was impaired in HepG2.2.15 cells, a stable HBV-expressing cell line, compared with its parental cell line HepG2. Altered miRNA expression profile, in particular the significant upregulation of miR-192, was observed in HepG2.2.15 cells. Additionally, ERCC3 and ERCC4, two key factors implicated in NER, were identified as targets of miR-192 and over-expressing miR-192 significantly inhibited cellular NER. These results indicated that persistent HBV infection might trigger NER impairment in part through upregulation of miR-192, which suppressed the levels of ERCC3 and ERCC4. It provides new insight into the effect of chronic HBV infection on NER and genetic instability in cancer.
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| Overall design |
A genome-wide miRNAs microarray was performed to identify differentially expressed miRNAs between HepG2.2.15, a stable HBV-expressing cell line, and its parental cell line HepG2.
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| Contributor(s) |
He X, Xie Q, Chang Y, Lin J |
| Citation(s) |
21672525 |
| Submission date |
Feb 28, 2011 |
| Last update date |
May 07, 2014 |
| Contact name |
Xing-Xing He |
| E-mail(s) |
healthhe@hotmail.com
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| Phone |
86-27-69378506
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| Organization name |
Tongji Hospital
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| Department |
Institute of Liver and Gastrointestinal Diseases
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| Lab |
Institute of Liver and Gastrointestinal Diseases
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| Street address |
1095# Jie-Fang Avenue
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430030 |
| Country |
China |
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| Platforms (1) |
| GPL8227 |
Agilent-019118 Human miRNA Microarray 2.0 G4470B (miRNA ID version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA138399 |
