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| Status |
Public on Aug 07, 2012 |
| Title |
Development of gene expression signatures for latent and clinical tuberculosis (TB) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
For further identify the differentiation between latent and clinical tuberculosis (TB), we employed whole genome microarray expression profiling to study genes with significant expression change in peripheral CD4+T cells between healthy control, latent tuberculosis (LTB) and clinical tuberculosis (TB). Our experiment included 4 groups: healthy donor (HD), latent TB1 (LTB1) with low IFN-gamma release level, latent TB2 (LTB2) with high IFN-gamma release level, and tuberculosis (TB) with high IFN-gamma release level. Human peripheral blood mononuclear cells were collected, from which CD4+T cells were isolated. Total RNA of each individuals of each group was extracted from peripheral CD4+T cells. One μg of RNA mixture, pooled equivalently by each individual total RNA of each group, was administrated microarray test. Compared with HD, through analyzing enriched-Gene Ontology (GO) terms and KEGG pathways of each group, we found peripheral CD4+T cells might had different ability for mycobacterium tuberculosis infection in LTB1, LTB2 and TB. Finally we detected that TNFSF13/APRIL and TNFSF13B/BAFF was significant up-regulation in both CD4+T cells and serum of TB by real time PCR and ELISA, respectively.
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| Overall design |
Peripheral CD4+ T cells were purified by positive selection using magnetic beads (BD IMagTM anti-human CD4 Particles-DM, BD Biosciences). Total RNA was extracted from CD4+ T cells (CD4-RNA) of LTB1, LTB2 and TB patients and healthy controls by RNeasy Mini Kit (QIAGEN).Then, RNA quality was checked and mixed with an equal quantity of each individual. The number of included-individuals in each group was 11 for HD, 11 for LTB1, 12 for LTB2 and 11 for TB.
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| Contributor(s) |
Liu K, Zhang Y, Yang Q, Yu Y, Hu S, Chen X, Liu H, Jin Q |
| Citation(s) |
22719887 |
| Submission date |
Mar 10, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
hyk liu |
| E-mail(s) |
liukailucky@yahoo.com.cn
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| Organization name |
IPB
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| Street address |
beijing
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| City |
beijing |
| ZIP/Postal code |
10000 |
| Country |
China |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA138233 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE27882_raw_data.txt.gz |
3.5 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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