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| Status |
Public on Sep 15, 2011 |
| Title |
Smad3 regulates the miR-200 family in gastric cancer |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
To find potential microRNA links between Smad3 and E-cadherin, we characterized the microRNA profiles of two gastric cancer cell lines: SNU484-LPCX, which does not express Smad3, and SNU484-Smad3, in which Smad3 is overexpressed. We found that miR-200 families, among other differentially expressed miRNAs, are overexpressed in SNU484-Smad3. Through subsequent studies, including silencing of Smad3 in SNU484-Smad3 and expression profiling of epithelial-mesenchymal markers and ZEB1/2, known repressors of E-cadherin, we found that Smad3 regulates miR-200 families at the transcriptional level, which regulate ZEB 1/2, known transcriptional repressors of E-cadherin, at the post-transcriptional level. This represents an important link between the TGF-beta signaling pathway and post-transcriptional regulation by miRNAs.
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| Overall design |
Examination of small RNA expression in 2 different cell lines (SNU484-LPCX, SNU484-Smad3).
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| Contributor(s) |
Kim D, Ahn S |
| Citation(s) |
22020340 |
| Submission date |
Mar 15, 2011 |
| Last update date |
Feb 21, 2019 |
| Contact name |
DeokHoon Kim |
| E-mail(s) |
coonya@gmail.com
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| Phone |
82-32-899-6546
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| Organization name |
Lee Gil Ya cancer and diabets institute
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| Lab |
lab of genomics
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| Street address |
7-45 songdo-dong yeonsu-ku
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| City |
incheon |
| ZIP/Postal code |
406-840 |
| Country |
South Korea |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA137775 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE27977_RAW.tar |
19.1 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE27977_readme.txt |
2.3 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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