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| Status |
Public on Mar 23, 2011 |
| Title |
Expression data from CH/LE Mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We are using genome-wide ChIP-seq with isoform-specific antibodies and chromatin from select tissues of mice challenged with different dietary conditions that enrich for specific SREBPs.
We show that hepatic SREBP-2 binds preferentially to two different gene-proximal motifs as well as SREBP-2 directly activates autophagy genes during cell sterol depletion, conditions known to induce both autophagy and nuclear SREBP-2 levels.
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| Overall design |
Gene expression profiling was carried out using the Mouse Gene 1.0 OST (Affymetrix) by hybridizing RNA from LE and Ch livers in triplicate at the Sanford-Burnham Genomics Core facility in Lake Nona Fl. Differential expression was then assessed using the Partek Genomics Suite (Partek Inc.) and cyberT.
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| Contributor(s) |
Seo Y, Jeon T, Chong H, Biesinger J, Xie X, Osborne TF |
| Citation(s) |
21459322 |
| Submission date |
Mar 22, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Jacob Biesinger |
| E-mail(s) |
jake.biesinger@gmail.com
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| Phone |
949 231 7587
|
| Organization name |
University of California, Irvine
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| Department |
Computer Science
|
| Lab |
Xiaohui Xie
|
| Street address |
4058 Donald Bren Hall, University of California Irvine
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| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92617 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE28084 |
Genome-wide Localization of SREBP-2 in Hepatic Chromatin Predicts a Novel Role in Autophagy |
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| Relations |
| BioProject |
PRJNA143127 |
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