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| Status |
Public on Mar 15, 2012 |
| Title |
Analysis of PGC-1alpha overexpression effects on the whole transcriptome in cultured skeletal muscle cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The transcriptional coactivator peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1α) is a chief activator of the mitochondrial and metatolic program in skeletal muscle (skm) and prevents atrophy. Here we tested whether PGC-1α overexpression could restructure the transcriptome and metabolism of cultured human skeletal myotubes, which display an athropic phenotype. An oligonucleotide microarray analysis was used to reveal PGC-1α effects on the whole transcriptome, and the possible impact on fuel metabolism reprogramming was examined. Fifty-three different genes displayed changed expression levels in response to PGC-1α: 42 upregulated and 11 downregulated. Main associations of gene ontologies (GO) for the upregulated genes were mitochondrial components and processes and this correlated with an increase in COX activity, an indicator of mitochondrial content. Palmitate and lactate oxidation to CO2 was enhanced, but not glucose oxidation. The other most significant GO associations of upregulated genes were chemotaxis and cytokine activity, and accordingly, several cytokines, including IL8, CXCL6, CCL5 and CCL8, were within top induced genes. Among the most regulated genes were also potential metabolic regulators of fatty acid and glucose storage. FITM1/FIT1 induction was associated with an increased number of lipid droplets with smaller area, while triglyceride levels were modestly increased in oleate-incubated cells. Downregulation of CALM1, the calcium-modulated δ subunit of phosphorylase kinase, was linked to inactivation of glycogen phosphorylase and greater accumulation of glycogen. The most upregulated gene was PVALB, which is also related to calcium signaling. In conclusion, only the deficient mitochondrial transcriptional program of cultured myotubes is rescued by PGC-1α. New PGC-1α gene targets and pathways arise, some of which may mediate its activating effects in processes such as lipid and carbohydrate storage and angiogenesis.
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| Overall design |
6 samples from 3 different skeletal muscle cell cultures were used: 3 were transfected with adenovirus containing PGC-1aplha and 3 with adenovirus containing GFP (control). The 3 PGC-1alpha samples were compared against the control samples.
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| Contributor(s) |
Mormeneo E, Gomez-Foix AM |
| Citation(s) |
22272266 |
| Submission date |
Mar 28, 2011 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Sergi Beltran |
| Organization name |
Universitat de Barcelona
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| Department |
Serveis Cientificotècnics
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| Lab |
Unitat de Bioinformàtica
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| Street address |
Baldiri Reixac 10
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| City |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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| Samples (6)
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| GSM698462 |
experiment 44, PGC-1alpha transduced |
| GSM698463 |
experiment 46, PGC-1alpha transduced |
| GSM698464 |
experiment 24, PGC-1alpha transduced |
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| Relations |
| BioProject |
PRJNA139647 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28206_RAW.tar |
6.3 Mb |
(http)(custom) |
TAR |
| GSE28206_non-normalized.txt.gz |
2.0 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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