|
| Status |
Public on Apr 14, 2011 |
| Title |
Gene expression analysis of the colon with and without DNA methyltransferase 1 (Dnmt1) inhibition |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We performed gene expression microarray to assess the effects of reduced DNA methylation on global gene expression. Gene expression profiles of colonic mucosa from mice with two different genotypes (Dnmt1LO/LO and Dnmt1LO/LO, TgVillacIGY) were compared. Dnmt1 expression is repressed only in the Dnmt1LO/LO, TgVillacIGY mice.
|
| |
|
| Overall design |
Total RNA was isolated from the colonic mucosa of four mouse, two biological replicates of each genotype in two technical relicates. Their gene expression profiles were obtained using the Illumina Ref-8 whole-genome expression BeadChip (MouseRef-8 v2.0). We processed our data using the Lumi Id and ReMOAT annotation data base (MouseWG-6 corresponding to GPL6887).
|
| |
|
| Contributor(s) |
Lee K, Oghamian S, Laird PW |
| Citation(s) |
28981717 |
| Submission date |
Apr 05, 2011 |
| Last update date |
Oct 04, 2019 |
| Contact name |
KwangHo Lee |
| E-mail(s) |
kwangho@usc.edu
|
| Phone |
323 828-0096
|
| Fax |
323 865-0158
|
| Organization name |
The University of Southern California
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Peter W. Laird
|
| Street address |
1441 Eastlake Ave NOR 6418
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA139085 |
Less...
More...