|
| Status |
Public on May 11, 2011 |
| Title |
Dynamic Chromatin Localization of Sirt6 Shapes Stress- and Aging- Related Transcriptional Networks (Illumina) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Sirtuins (Sirt) are a family of enzymes that modify chromatin and other proteins to affect gene activity. Loss of Sirt6 leads to a progeria-like phenotype in mice, but the target of SIRT6 action has been elusive. Here we show that Sirt6 binds to thousands of gene promoters in a stress-inducible fashion, guided by the stress-responsive transcription factor NF-κB.
Chromatin profiling by ChIP-chip analysis of Sirt6 and NF-KB component RelA combined with expression array data of wildtype, Sirt6 knockout and Sirt6 RelA double knockout cells demonstrates that RelA recruits Sirt6 to NF-KB targets in response to TNF-a induction and that many of these targets are important for senescence and aging.
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| |
|
| Overall design |
comparison of wild type, Sirt6-/- and Sirt6-/- RelA-/- MEF cells
|
| |
|
| Contributor(s) |
Tiara L K, Nicole A R, Angela R W, Kun Q, Stephan R Q, Howard Y C |
| Citation(s) |
21738489 |
| Submission date |
Apr 13, 2011 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Kun Qu |
| E-mail(s) |
kqu@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Dermatology
|
| Lab |
Howard Chang
|
| Street address |
269 Campus Dr. CCSR 2150
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (12)
|
|
| This SubSeries is part of SuperSeries: |
| GSE28641 |
Dynamic Chromatin Localization of Sirt6 Shapes Stress- and Aging- Related Transcriptional Networks |
|
| Relations |
| BioProject |
PRJNA143009 |
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