|
| Status |
Public on Feb 16, 2012 |
| Title |
Gene expression analysis of ovarian tumors from mice with knockout of DICER and PTEN |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The cell of origin of serious ovarian cancer is unknown. To create a mouse model for this lethal cancer and identify early cancer biomarkers, we conditionally deleted both Dicer (essential for microRNA biosynthesis) and Pten (a negative regulator of the PI3K pathway) in the female reproductive tract. Beginning at ~3-5 months, these Dicer/Pten mutant mice develop high-grade serious carcinomas that initiate in the stroma of the fallopian tube through a mesenchymal-to-epithelial transition (MET), subsequently envelop the ovary, and then metastasize throughout the peritoneum, resulting in ascites and 100% lethality by 13 months. The fallopian tube cancers demonstrate upregulation of genes encoding known and novel secreted proteins that are potential biomarkers. This study uncovers a new paradigm for the initiation of high-grade serous ovarian cancer.
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| Overall design |
RNA was isolated from the fallopian tube cancers of independent DKO mice and normal fallopian tubes of control mice and subjected to mRNA expression analysis using an Illumina platform (MouseWG-6 v2 Expression BeadChip).
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| Contributor(s) |
Creighton C, Matzuk M, Kim J, Hawkins S |
| Citation(s) |
22331912 |
| Submission date |
Apr 19, 2011 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (6)
|
| GSM711456 |
normal mouse fallopian tube oviduct, biological rep1 |
| GSM711457 |
normal mouse fallopian tube oviduct, biological rep2 |
| GSM711458 |
normal mouse fallopian tube oviduct, biological rep3 |
| GSM711459 |
early tumors from fallopian tubes of Dicer/PTEN knockout mice, biological rep 1 |
| GSM711460 |
early tumors from fallopian tubes of Dicer/PTEN knockout mice, biological rep 2 |
| GSM711461 |
early tumors from fallopian tubes of Dicer/PTEN knockout mice, biological rep 3 |
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| This SubSeries is part of SuperSeries: |
| GSE28979 |
Gene expression profiles of serous ovarian cancer in human and mouse |
|
| Relations |
| BioProject |
PRJNA143067 |
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