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| Status |
Public on Jul 11, 2011 |
| Title |
Androgen receptor cistrome in breast cancer cell line MDA-MB-453 with 5a-dihydrotestosterone (DHT) stimulation |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Androgen receptor (AR) is expressed in 60-70% of breast cancers independent of estrogen receptor (ER) expression, however its function in breast cancer is largely unknown. Our study identified the high level of AR in ERâ/HER2+ breast tumors and andorgen and AR greatly stimulated growth of MDA-MB-453 breast cancer cells. To define the genome-wide AR binding sites, we performed AR ChIP-seq using MDA-MB-453 breast cancer cells followig stimulation of DHT. We also identified FOXA1 is a crucial AR cofactor in MDA-MB-453 cells and the FOXA cistrome showed signaficant overlap with AR at both early and late time points of DHT stimulation.
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| Overall design |
AR ChIP was performed in MDA-MB-453 breast cancer cells following 5a-dihydrotestosterone (DHT) stimulation for 4h and 16h respectively. FOXA1 ChIP-seq was performed after 4h DHT stimulation in MDA-MB-453 cells.
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| |
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| Contributor(s) |
Ni M, Chen Y, Brown M |
| Citation(s) |
21741601 |
| Submission date |
Apr 21, 2011 |
| Last update date |
Jun 11, 2013 |
| Contact name |
Yiwen Chen |
| E-mail(s) |
ychen26@mdanderson.org
|
| Organization name |
University of Texas MD Anderson Cancer Center
|
| Department |
Bioinformatics and Computational Biology
|
| Lab |
Yiwen Chen
|
| Street address |
1400 Pressler Street
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL14761 |
Helicos HeliScope (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE28789 |
Breast cancer cell line MDA-MB-453 response to DHT |
|
| Relations |
| BioProject |
PRJNA143095 |
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