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| Status |
Public on Apr 01, 2017 |
| Title |
Identification of GLI1 direct and indirect target genes in human pancreatic cancer cell line Panc-1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Panc-1GLI1ER and Panc-1ER were established from human pancreatic cancer cell line Panc-1. Panc-1GLI1ER cells express a chimeric transgene composed of an enhanced green fluorescent protein (EGFP)-tagged version of the amino-terminal half of GLI1, fused to the AF2 domain of the mouse estrogen receptor 1(Esr1) cDNA. On the other hand, Panc-1ER harbor only EGFP-tagged AF2 domain.
To identify GLI1 direct target genes, total RNAs were purified from the cells before and 24 hours after the beta-estradiol (E2) treatment.
Gene expression profiles were analyzed by AGILENT human 4x44 cDNA microarray.
Keywords: Expression profiling by genome tiling array
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| Overall design |
GLI1 direct and indirect target genes were identified in Panc-1 cells. Gene expression profiles of Panc-1GLI1ER and Panc-1ER cells were analyzed by AGILENT human 4x44 cDNA microarray before and 24 hours after treatment of beta-estradiol (E2) at doses of 10nM.
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| Contributor(s) |
Inaguma S, Kasai K, Ikeda H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 28, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Shingo Inaguma |
| Organization name |
Aichi Medical University School of Medicine
|
| Department |
Department of Pathology and Promoting Center for Clinical Research
|
| Street address |
Nagakute
|
| City |
Aichi |
| ZIP/Postal code |
480-1195 |
| Country |
Japan |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (4)
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| GSM712081 |
Panc-1GLI1ER_10nM beta-estradiol (E2)_24hrs |
|
| Relations |
| BioProject |
PRJNA140661 |
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