|
| Status |
Public on Aug 02, 2011 |
| Title |
Replication initiation sites in cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Reads from massively parallel sequencing of RNA primed, short nascent strands from asynchronously growing cancer cells (K562, MCF7, HCT116).
|
| |
|
| Overall design |
Newly replicated DNA was isolated based on size (400-800 bp) and the presence of a short RNA stretch at the 5' end using lambda exonuclease. Purified nascent strands were analyzed using massively parallel sequencing. Sheared genomic DNA was sequenced as a control.
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| |
|
| Contributor(s) |
Aladjem MI, Fu H, Martin MM, Ryan M, Kim R, Smith OK |
| Citation(s) |
21813623, 24419320, 25879486, 27272143 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| ZIA BC010411 |
Initiation of DNA Replication in Mammalian Cells |
National Cancer Institute - Division of Basic Sciences |
Mirit I Aladjem |
|
| Submission date |
Apr 28, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Mirit I Aladjem |
| E-mail(s) |
aladjemm@mail.nih.gov
|
| Phone |
301-435-2848
|
| Organization name |
National Cancer Institute
|
| Department |
DNA Replication Group
|
| Street address |
37 Convent Dr. Building 37, Room 5068D
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (7)
|
|
| Relations |
| SRA |
SRP006514 |
| BioProject |
PRJNA140633 |
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