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| Status |
Public on Oct 01, 2011 |
| Title |
Calcineurin/NFAT signaling inhibits myeloid hematopoiesis in mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Nuclear factor of activated T cells (NFAT) comprises a family of transcription factors that regulate T cell development, activation and differentiation. NFAT signaling can also mediate granulocyte and DC activation, but it is unknown whether NFAT influences their development from progenitors. Here we report a novel role for calcineurin/NFAT signaling as a negative regulator of myeloid hematopoiesis. Reconstituting lethally-irradiated mice with hematopoietic stem cells expressing an NFAT-inhibitory peptide resulted in enhanced development of the myeloid compartment. Culturing bone marrow cells with Flt3-L and Cyclosporin A, which inhibits NFAT signaling, increased numbers of differentiated DC. Global gene expression analysis of untreated DC and NFAT-inhibited DC revealed differential expression of transcripts that regulate cell cycle and apoptosis. Thus, calcineurin/NFAT signaling negatively regulates myeloid lineage development. The finding that NFAT acts as a negative regulator of myeloid development provides novel insight in understanding immune responses during treatment with calcineurin/NFAT inhibitors as Cyclosporin A.
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| Overall design |
bone marrow cells from C57B/6 mice were stimulated in Flt3-L suplemented media in presence or absence of calcineurin/NFAT inhibitor Cyclosporin A, samples in 3 biological replicates
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| Contributor(s) |
Fric J, Lim C, Koh E, Hofmann B, Chen J, Isa SA, Mortellaro A, Ruedl C, Ricciardi-Castagnoli P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 05, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
jinmiao chen |
| Organization name |
Singapore Immunology Network
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| Street address |
Biopolis
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| City |
Singapore |
| ZIP/Postal code |
138648 |
| Country |
Singapore |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (27)
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| GSM720744 |
bone marrow cells, unstimulated, rep1 |
| GSM720745 |
bone marrow cells, unstimulated, rep2 |
| GSM720746 |
bone marrow cells, unstimulated, rep3 |
| GSM720747 |
bone marrow cells, 12h stimulated with Flt3-L rep1 |
| GSM720748 |
bone marrow cells, 12h stimulated with Flt3-L rep2 |
| GSM720749 |
bone marrow cells, 12h stimulated with Flt3-L rep3 |
| GSM720750 |
bone marrow cells, 24h stimulated with Flt3-L rep1 |
| GSM720751 |
bone marrow cells, 24h stimulated with Flt3-L rep2 |
| GSM720752 |
bone marrow cells, 24h stimulated with Flt3-L rep3 |
| GSM720753 |
bone marrow cells, 48h stimulated with Flt3-L rep1 |
| GSM720754 |
bone marrow cells, 48h stimulated with Flt3-L rep2 |
| GSM720755 |
bone marrow cells, 48h stimulated with Flt3-L rep3 |
| GSM720756 |
bone marrow cells, 11 days stimulated with Flt3-L rep1 |
| GSM720757 |
bone marrow cells, 11 days stimulated with Flt3-L rep2 |
| GSM720758 |
bone marrow cells, 11 days stimulated with Flt3-L rep3 |
| GSM720759 |
bone marrow cells, 12h stimulated with Flt3-L, treated with Cyclosporin A rep1 |
| GSM720760 |
bone marrow cells, 12h stimulated with Flt3-L, treated with Cyclosporin A rep2 |
| GSM720761 |
bone marrow cells, 12h stimulated with Flt3-L, treated with Cyclosporin A rep3 |
| GSM720762 |
bone marrow cells, 24h stimulated with Flt3-L, treated with Cyclosporin A rep1 |
| GSM720763 |
bone marrow cells, 24h stimulated with Flt3-L, treated with Cyclosporin A rep2 |
| GSM720764 |
bone marrow cells, 24h stimulated with Flt3-L, treated with Cyclosporin A rep3 |
| GSM720765 |
bone marrow cells, 48h stimulated with Flt3-L, treated with Cyclosporin A rep1 |
| GSM720766 |
bone marrow cells, 48h stimulated with Flt3-L, treated with Cyclosporin A rep2 |
| GSM720767 |
bone marrow cells, 48h stimulated with Flt3-L, treated with Cyclosporin A rep3 |
| GSM720768 |
bone marrow cells, 11 days stimulated with Flt3-L, treated with Cyclosporin A rep1 |
| GSM720769 |
bone marrow cells, 11 days stimulated with Flt3-L, treated with Cyclosporin A rep2 |
| GSM720770 |
bone marrow cells, 11 days stimulated with Flt3-L, treated with Cyclosporin A rep3 |
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| Relations |
| BioProject |
PRJNA140321 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29094_RAW.tar |
117.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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