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| Status |
Public on May 11, 2011 |
| Title |
Deficiency in the multicopy Sycp3-like X-linked genes Slx and Slxl1 causes major defects in spermatid differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The gene Sly is present in multiple copies on the mouse Y chromosome and encodes a protein that is required for the epigenetic regulation of postmeiotic sex chromosome expression. The X chromosome carries two multicopy genes related to Sly: Slx and Slxl1. Here we investigate the role of Slx/Slxl1 using transgenically-delivered small interfering RNAs to disrupt their function. We show that Slx and Slxl1 are important for normal sperm differentiation and male fertility. Slx/Slxl1 deficiency leads to delay in spermatid elongation and sperm release. A high proportion of delayed spermatids are eliminated via apoptosis, with a consequent reduced sperm count. The remaining spermatozoa are abnormal with impaired motility and fertilizing abilities. Microarray analyses reveal that Slx/Slxl1 deficiency affects the metabolic processes occurring in the spermatid cytoplasm but does not lead to a global perturbation of sex chromosome expression; this is in contrast with the effect of Sly deficiency which leads to an up-regulation of X and Y chromosome genes.
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| Overall design |
Total RNA isolated from separated round spermatids of young adult males (8 weeks postpartum) with shSLX or shSLY targeted knockdown, compared to age- and strain-matched controls.
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| Contributor(s) |
Cocquet J, Ellis PJ, Yamauchi Y, Riel JM, Karacs TP, Rattigan A, Ojarikre OA, Affara NA, Ward MA, Burgoyne PS |
| Citation(s) |
20739462 |
| Submission date |
May 10, 2011 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Peter James Ellis |
| E-mail(s) |
p.j.i.ellis@kent.ac.uk
|
| Phone |
07808167834
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| Organization name |
University of Kent
|
| Department |
School of Biosciences
|
| Street address |
Stacey Building
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| City |
Canterbury |
| State/province |
Kent |
| ZIP/Postal code |
CT2 7NJ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA140039 |
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