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Series GSE2933 Query DataSets for GSE2933
Status Public on Jul 15, 2005
Title Aldosterone-responsive genes in kidney cortical collecting duct cell line, mpkCCDc14
Organism Mus musculus
Experiment type Expression profiling by array
Summary Aldosterone is arguably the single most important hormone implicated in the control of blood pressure and extracellular fluid volume in mammals. It acts primarily by increasing the rate of transepithelial sodium transport in the kidney tubules. Several monogenetic defects resulting in hypertension have now been attributed to abnormalities in sodium handling within the aldosterone-sensitive distal nephron, where the epithelial sodium channel, ENaC, constitutes the rate-limiting step of sodium transport. To date, the transcription-dependent aldosterone-signaling pathway between receptor and membrane transport effectors, remains incompletely understood. The broad aim of our study is to explore these intracellular signaling pathways that are critical to the functioning of sodium channels. Microarray analysis in an aldosterone-responsive kidney cortical collecting duct cell line (mpkCCDc14), allowed us to identify many potential aldosterone-regulated transcripts. The present study describes the identification, and possible physiological relevance of various aldosterone-regulated transcripts. The results of this study promise to extend our understanding of the molecular basis of aldosterone-regulated sodium transport in kidney epithelia, and provide approaches for regulating this process in disease states such as congestive heart failure and hypertension.
Keywords: hormone effect
 
Overall design mpkCCD Cells were treated with 10-6M aldosterin or ethanol for 1 or 6 hrs. Total RNAs were then isolated and cDNA was made and purified and recovered. The Cy3 or Cy5 was then coupled with cDNA from control or drug-treated cells, respectively. The labeled cDNA was hybridized with microarray slides. 4 arrays for each experimental conditions.
 
Contributor(s) Zhang T, Pearce D
Citation(s) 16216878
Submission date Jul 13, 2005
Last update date Mar 16, 2012
Contact name Ting Ting Zhang
E-mail(s) tizst0@yahoo.com
Phone 617-855-2179
Fax 617-855-2179
Organization name Harvard University
Department Mclean Hospital
Lab bioorgnic and natural product
Street address 115 Millman st
City Belmont
State/province MA
ZIP/Postal code 02478
Country USA
 
Platforms (1)
GPL2623 Qiagen/Operon version 2.0 mouse oligonucleotide
Samples (8)
GSM64041 Aldosterone-responsive genes in kidney cortical collecting duct cell line, mpkCCDc14 (1 hour treatment)
GSM64042 Aldosterone-responsive genes in kidney cortical collecting duct mpkCCDc14 (1 hour treatment)
GSM64043 Aldosterone-responsive genes in kidney cortical collecting duct cell mpkCCDc14 (1 hour treatment)
Relations
BioProject PRJNA92439

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