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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 20, 2011 |
| Title |
Sequencing of microRNA in spermatogonial stem cell enriched cell populations. |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
MicroRNAs (miRs) play a key role in the control of gene expression in a wide array of tissue systems where their functions include the regulation of self-renewal, cellular differentiation, proliferation, and apoptosis. However, the functional importance of individual miRs in controlling spermatogonial stem cell (SSC) homeostasis has not been investigated. Using high-throughout sequencing, we profiled the expression of miRs in the Thy1+ testis cell population, which is highly enriched for SSCs, and the Thy1- cell population, composed primarily of testis somatic cells. In addition, we profiled the global expression of miRs in cultured germ cells, also enriched for SSCs. Our results demonstrate that miR-21, along with miR-34c, -182, -183, -146a, -465a-3p, -465b-3p, -465c-3p, and -465c-5p are preferentially expressed in the Thy1+ SSC-enriched population, as compared to Thy1- somatic cells, and we further observed that Thy1+ SSC-enriched testis cells and SSC-enriched cultured germ cells share remarkably similar miR expression profiles.
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| Overall design |
Spermatogonial Stem Cell enriched cell populations (freshly isolated and short-term cultured) and somatic cell populations were isolated from C57B/L6 mouse donors and subjected to small RNA isolation and sequencing.
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| Contributor(s) |
Niu Z, Brinster RL |
| Citation(s) |
21768389 |
| Submission date |
May 28, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Zhiyv Niu |
| E-mail(s) |
zhiyv.niu@gmail.com
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| Organization name |
University of Pennsylvania
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| Department |
Animal Biology
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| Lab |
Reproductive Biology
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| Street address |
One baylor plaza, 506E
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| City |
houston |
| State/province |
tx |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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| Samples (5)
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| Relations |
| SRA |
SRP006999 |
| BioProject |
PRJNA141359 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29613_RAW.tar |
1.1 Mb |
(http)(custom) |
TAR (of TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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