|
| Status |
Public on Jan 01, 2012 |
| Title |
Zfx controls self-renewal of human embryonic stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Previously, we identified the transcription factor Zfx as a key regulator of self-renewal in murine ESCs. Here we extend those findings to human ESCs. Zfx knockdown in hESCs hindered clonal growth and decreased colony size after serial replating. Zfx overexpression enhanced clone formation, increased colony size and decreased expression of differentiation-related genes in human ESCs. Zfx-overexpressing hESCs resisted spontaneous differentiation but could be directed to differentiate into endodermal and neural cell fates when provided with the appropriate cues. Thus, Zfx acts as a molecular rheostat regulating the balance between self-renewal and differentiation in hESCs, revealing the close evolutionary conservation of the self-renewal mechanisms in murine and human ESCs.
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| Overall design |
Human embryonic stem cells with over expression of ZFX were compared to the original cell line (H9) and a clonally derived wild-type cell line (clone 3)
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| |
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| Contributor(s) |
Tomishima MJ, Reizis B, Chambers SM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 07, 2011 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Stuart M Chambers |
| Organization name |
Sloan-Kettering Institute
|
| Street address |
1270 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
|
| Samples (8)
|
| GSM738205 |
Embryonic stem cells Rep1 |
| GSM738206 |
Embryonic stem cells Rep2 |
| GSM738207 |
ZFX over expressing embryonic stem cells Clone 2 Rep1 |
| GSM738208 |
ZFX over expressing embryonic stem cells Clone 2 Rep2 |
| GSM738209 |
Control embryonic stem cell Clone 3 Rep1 |
| GSM738210 |
Control embryonic stem cell Clone 3 Rep2 |
| GSM738211 |
ZFX over expressing embryonic stem cells Clone 5 Rep1 |
| GSM738212 |
ZFX over expressing embryonic stem cells Clone 5 Rep2 |
|
| Relations |
| BioProject |
PRJNA141059 |
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