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| Status |
Public on Jun 16, 2011 |
| Title |
Cancer-related epigenome changes associated with reprogramming to induced pluripotent stem cells (expression data) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The ability to induce pluripotent stem cells from committed, somatic human cells provides tremendous potential for regenerative medicine. However, there is a defined neoplastic potential inherent to such reprogramming that must be understood and may provide a model for understanding key events in tumorigenesis. Using genome-wide assays, we identify cancer-related epigenetic abnormalities that arise early during reprogramming and persist in induced pluripotent stem cell (iPS) clones. These include hundreds of abnormal gene silencing events, patterns of aberrant responses to epigenetic-modifying drugs resembling those for cancer cells, and presence in iPS and partially reprogrammed cells of cancer-specific gene promoter DNA methylation alterations. Our findings suggest that by studying the process of induced reprogramming, we may gain significant insight into the origins of epigenetic gene silencing associated with human tumorigenesis, and add to means of assessing iPS for safety.
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| Overall design |
Direct expression comparison of iPS lines, cultured stem cell lines and normal differentiated cells. Re-expression experiments with 5-aza-2′-deoxycytidine (AZA) and trichostatin A (TSA) to identify hypermethylated genes.
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| Contributor(s) |
Van Neste L, Ohm J, Baylin SB |
| Citation(s) |
20841480 |
| Submission date |
Jun 09, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Leander Van Neste |
| Organization name |
Ghent University
|
| Department |
Molecular Biotechnology
|
| Lab |
Bioinformatics and Computational Genomics
|
| Street address |
Coupure Links 653
|
| City |
Ghent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
| |
|
| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (31)
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| This SubSeries is part of SuperSeries: |
| GSE29873 |
Cancer-related epigenome changes associated with reprogramming to induced pluripotent stem cells |
|
| Relations |
| BioProject |
PRJNA155223 |
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