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Series GSE30169 Query DataSets for GSE30169
Status Public on Jul 20, 2011
Title Expression of human aortic endothelial cells treated with or without oxidized phospholipids (II)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Oxidized phospoholipids are a pro-inflammatory component of minimally modified lipoproteins that get trapped in the subendothelial space of atherosclerotic plaques of large arteries. To model the response of endothelial cells in a pro-atherosclerotic enviroment we measured the expression in primary endothelial cells with and without treatment with oxidized phsopolipids from 96 genetically identical donors of anonymous origin.
These samples are an extention of samples of similar origin deposited in GSE20060
 
Overall design Primary human aortic endothelial cells were collected from aortic heart explants of heart transplant donors and cultured in standard cell culture condiditions. At confluence, cells were treated with or without 40 ug/ml oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphatidylcholine (Ox-PAPC) for 4 hours in media 199 containing 1% fetal bovine serum. After 4 hours, the cells were collected for RNA extraction and quantification of transcripts via microarrays.

Application of electronic probe mask (HT_U133Amsk.cdf) on raw data.
 
Contributor(s) Romanoski CE, Lusis A
Citation(s) 21737788, 23667179
Submission date Jun 23, 2011
Last update date Aug 23, 2019
Contact name Casey Romanoski
Organization name UCSD
Street address 9500 Gliman Dr.
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platforms (1)
GPL3921 [HT_HG-U133A] Affymetrix HT Human Genome U133A Array
Samples (629)
GSM752709 HumanAorticEndothelialCells_Individual501_controlTreatment_replicate1
GSM752710 HumanAorticEndothelialCells_Individual501_controlTreatment_replicate2
GSM752711 HumanAorticEndothelialCells_Individual501_OxPAPCTreatment_replicate1
Relations
BioProject PRJNA143911

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30169_HT_U133Amsk.cdf.gz 6.0 Mb (ftp)(http) CDF
GSE30169_RAW.tar 1.7 Gb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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