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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 13, 2014 |
| Title |
The ets transcription factor ELF5 suppresses the estrogen sensitive phenotype and contributes to antiestrogen resistance in luminal breast cancer. [mouse] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The ets transcription factor ELF5 specifies the differentiation of mammary progenitor cells to establish the milk-secreting lineage. ER- and poor prognosis basal breast cancers arise from this progenitor cell and these cancers express high levels of Elf5. Knockdown of ELF5 expression in basal breast cancer cell lines, or forced expression in luminal breast cancer cell lines, resulted in reduced cell proliferation. Transcript profiling and chromatin immunoprecipitation revealed that the transcriptional activity of ELF5 specified the gene expression patterns that distinguish basal from luminal breast cancer, including suppression of FOXA1, GATA3 and ER, key estrogen-action genes. Tamoxifen treatment of luminal MCF7 cells upregulated Elf5 expression and cells that acquired resistance to Tamoxifen became dependent on ELF5 for proliferation. ELF5 is a regulator of breast cancer cell proliferation, transcriptionally specifies the basal molecular subtype and is utilised by ER+ breast cancer cells to escape proliferative arrest caused by Tamoxifen.
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| Overall design |
Elf5 was induced via doxycycline treated PyMT mouse tumours, in triplicate
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| Contributor(s) |
Kalyuga M, Gallego-Ortega D, Lee HJ, Cowley MJ, Caldon L, Stone A, Allerdice SL, Valdes-Mora F, Launchbury R, Statham AL, Armstrong N, Alles MC, Young A, Egger A, Au W, Piggin CL, Evans CJ, Brummer T, Kaplan W, Gee JM, Nicholson RI, Sutherland RL, Swarbrick A, Naylor MJ, Clark SJ, Carroll JS, Ormandy CJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 05, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Mark Cowley |
| E-mail(s) |
m.cowley@garvan.org.au
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| Organization name |
Garvan Institute of Medical Research
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| Department |
Genome Informatics & Clinical Genomics
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| Lab |
Dinger lab
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| Street address |
384 Victoria St.
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| City |
Darlinghurst |
| State/province |
NSW |
| ZIP/Postal code |
2010 |
| Country |
Australia |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA154829 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30406_RAW.tar |
23.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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