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| Status |
Public on Dec 12, 2012 |
| Title |
Whole mouse genome analysis of LCMV-specific CD4 and CD8 T cells throughout acute and chronic LCMV infection. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
CD4 and CD8 T cells display functional defects during chronic infection such as loss of certain cytokines. Recent studies have suggested that CD4 T cells may actually gain other functions, however. Here, we analyzed gene expression profiles from LCMV-specific CD4 and CD8 T cells throughout the response to either acute LCMV or chronic LCMV infection. This alllowed us to identify CD4-specific changes during chronic infection compared to acute infection but also revealed shared core regulators between CD4 and CD8 T cells.
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| Overall design |
LCMV-specific CD4 and CD8 T cells were isolated 6, 8, 15 and 30 days post infection with LCMV Armstrong or LCMV clone 13. Naïve CD4 and CD8 T cells were also isolated from naïve mice as comparisons. Four replicates of each sample were hybridized. The only exception is LCMV-specific CD4 T cells isolated 6 days post infection with LCMV-Arm where only three replicates were hybridized.
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| Contributor(s) |
Crawford A, Angelosanto JM, Wherry EJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 06, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Alison Crawford |
| E-mail(s) |
alisoncrawford61@hotmail.com
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| Organization name |
University of Pennsylvania
|
| Department |
Microbiology
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| Street address |
421 Curie Blvd, BRB
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (71)
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| Relations |
| BioProject |
PRJNA143533 |
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