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| Status |
Public on Jul 08, 2011 |
| Title |
MOG-encoding DNA vaccines ameliorate EAE and display neuroprotective effects in treated mice summary |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Vaccination with naked DNA encoding myelin basic protein represents a promising therapeutic strategy in multiple sclerosis (MS). In this study, we assessed the potential of vaccination with a DNA construct coding for the myelin oligodendrocyte glycoprotein (MOG), an important candidate autoantigen in MS, to induce tolerance and protect against experimental autoimmune encephalomyelitis (EAE). Herein, we demonstrated that MOG-DNA vaccination reduced the clinical and histopathological signs of EAE when administered in both prophylactic and therapeutic settings. Further mechanistic experiments revealed that the protective effects of MOG-DNA vaccines were associated with a reduction of antigen-specific Th1 and Th17 cellular immune responses and expansion of regulatory T cells in periphery, and up-regulation in the central nervous system of genes encoding neurotrophic factors and proteins involved in remyelination. These results may set the rationale for the use of MOG-based DNA vaccines to induce tolerance in MS patients.
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| Overall design |
We analyzed brain and spinal cord samples from five treated and five control mice
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| Contributor(s) |
Fissolo N, Costa C, Nurtdinov R, Bustamante M, Llombart V, Mansilla M, Espejo C, Montalban X, Comabella M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 07, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Ramil Nurtdinov |
| E-mail(s) |
ramil.nurtdinov@crg.eu
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| Organization name |
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
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| Department |
Bioinformatics and Genomics
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| Lab |
Computational Biology of RNA Processing
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| Street address |
Dr. Aiguader 88
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| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA143471 |
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