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| Status |
Public on Oct 31, 2011 |
| Title |
Changes in DNA methylation In cultered WI-38 cells over passages |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
|
| Summary |
We used Illumina infinium methylation array analysis to document spontaneous DNA methylation changes in human fibroblasts growing in long term culture. Starting with a culture derived from a single cell we compared the methylation profile of late passage clones with that of the initial culture, allowing unambiguous identification of methylation changes.
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| |
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| Overall design |
Bisulphite converted DNA from the 5 samples were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2
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| |
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| Contributor(s) |
Eden A, Ron-Bigger S, Bocker M, Michlin D, Isaac S, Lyko F |
| Citation missing |
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| Submission date |
Jul 13, 2011 |
| Last update date |
Jan 02, 2015 |
| Contact name |
Amir Eden |
| E-mail(s) |
eden@vms.huji.ac.il
|
| Phone |
7226584981
|
| Fax |
7226585417
|
| Organization name |
Hebrew University
|
| Department |
Cell & Developmental Biology
|
| Street address |
Givat Ram
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
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| Platforms (1) |
| GPL8490 |
Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA144637 |
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