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| Status |
Public on Nov 05, 2011 |
| Title |
mRNA expression data of tumor samples with/without IDH1/2 mutations and of controls |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We determined differentially expressed genes between tumor samples with and without IDH1/2 mutations, and between tumors with IDH1/2 mutations and controls.
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| Overall design |
A total of 21 tumours which include 6 enchondromas and 10 chondrosarcomas (3 grade I, 7 grade II) of Ollier disease, as well as 1 enchondroma and 4 chondrosarcomas grade II of solitary tumors, and 6 controls (growth plate and cartilage) were selected for expression analysis. Sample preparation, RNA isolation, synthesis of cDNA, cRNA amplification, hybridization of cRNA onto the Illumina Human-6 v3.0 Expression BeadChips, microarray data preprocessing, quality control, and detection of differential expression were performed as described previously (PMID: 21372215, PMID: 21584901). We determined differential expression between tumor samples without detectable IDH1 or IDH2 mutation (n=3) and samples with IDH1 or IDH2 mutations (n=18), and between samples with IDH1 or IDH2 mutation and controls (n=6). Probes with Benjamini and Hochberg False discovery rate-adjusted P-values (adjP) < 0.05 and a log fold change (logFC) > 0.1 were considered to be significantly differentially expressed.
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| Contributor(s) |
Pansuriya TC, Bovee J, Oosting J, Kuijjer ML |
| Citation(s) |
22057234 |
| Submission date |
Jul 21, 2011 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Twinkal Chandubhai Pansuriya |
| E-mail(s) |
t.c.pansuriya@lumc.nl
|
| Phone |
0031715266528
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| Organization name |
LUMC
|
| Department |
Pathology
|
| Street address |
Albinusdreef 2
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| City |
Leiden |
| State/province |
Zuid-Holland |
| ZIP/Postal code |
2333ZA |
| Country |
Netherlands |
| |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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| Samples (27)
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| This SubSeries is part of SuperSeries: |
| GSE30844 |
Somatic mosaic IDH1 and IDH2 mutations are associated with enchondroma and spindle cell haemangioma in Ollier disease and Maffucci syndrome |
|
| Relations |
| BioProject |
PRJNA154559 |
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