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| Status |
Public on Jul 27, 2011 |
| Title |
Role of miR-19b and its target mRNAs on 5-fluorouracil resistance in colon cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Despite the efforts in defining the molecular mechanisms for the drug resistance in colorectal cancers, little is known about the roles of microRNAs. With microarray containing 723 microRNAs, we examined effect of 5-fluorouracil (5-FU) on the microRNA expression. Respond to 5-FU, we identify two microRNAs, miR-19b and miR-21, that were differentially expressed in 5-FU resistant colon cancer cells derived from KM12C and DLD-1.
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| Overall design |
DLD-1, DLD-1/R, KM12C, and KM12C/R cells were plated at 1 × 105 cells/well. After pre-culture, cells were treated with 60 uM of 5-FU for 72 h. This was the same condition as the analysis of cell cycle. RNAs were collected before (0 h) and after the treatment of 5-FU (72 h).
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| Citation(s) |
22382630 |
| Submission date |
Jul 25, 2011 |
| Last update date |
Jul 21, 2014 |
| Contact name |
Toshihito Tanahashi |
| E-mail(s) |
tana@basic.med.tokushima-u.ac.jp
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| Organization name |
Tokushima University
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| Street address |
Kuramoto-cho 3-18-15
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| City |
Tokushima-city |
| ZIP/Postal code |
7708503 |
| Country |
Japan |
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| Platforms (1) |
| GPL8227 |
Agilent-019118 Human miRNA Microarray 2.0 G4470B (miRNA ID version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA144233 |
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