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Series GSE30997

Query DataSets for GSE30997

Status

Public on Jan 20, 2012

Title

[E-MTAB-652] CID island ChIP

Organism

Drosophila melanogaster

Experiment type

Genome binding/occupancy profiling by high throughput sequencing

Summary

The centromere-specific Histone H3-variant CENH3 (also known as CENP-A) is considered to be an epigenetic mark for establishment and propagation of centromere identity. Pulse-induction of CENH3 (Drosophila CID) in Schneider S2 cells incorporates into noncentromeric regions and generates CID islands that resist clearing from chromosome arms for multiple cell generations. We demonstrate that CID islands represent functional ectopic kinetochores, which are non-randomly distributed on the chromosome and display a preferential localization near telomeres and pericentric heterochromatin in transcriptionally silent, intergenic chromatin domains. Although overexpression of heterochromatin protein 1 (HP1) or increasing Histone acetylation interferes with CID islands formation on a global scale, induction of a locally defined region of synthetic heterochromatin by targeting HP1-LacI fusions to stably integrated Lac Operator arrays produces a proximal hotspot for CID islands formation. These data suggest that the characteristics of regions bordering heterochromatin promote de novo kinetochore assembly and thereby contribute to centromere identity.

ArrayExpress Release Date: 2011-07-15

Person Roles: submitter
Person Last Name: Diehl
Person First Name: Sarah
Person Mid Initials:
Person Email: diehl@immunbio.mpg.de
Person Phone: (+49) 761 5108 795
Person Address: Stuebeweg 51, 79108 Freiburg im Breisgau, Germany
Person Affiliation: Max-Planck-Institute for Immunobiology and Epigenetics

Person Roles: investigator
Person Last Name: Heun
Person First Name: Patrick
Person Mid Initials:
Person Email: heun@immunbio.mpg.de
Person Phone: (+49) 761 5108 717
Person Address: Stuebeweg 51, 79108 Freiburg im Breisgau, Germany
Person Affiliation: Max-Planck-Institute for Immunobiology and Epigenetics

Publication Title: Heterochromatin boundaries are hotspots for de novo kinetochore formation.
Publication Author List: Agata Olszak, Dominic van Essen, Antonio J. Pereira, Sarah Diehl, Thomas Manke, Helder Maiato, Simona Saccani and Patrick Heun

Overall design

Experimental Design: translational_design
Experimental Design: in_vitro_design
Experimental Design: ChiP-seq
Experimental Factor Name: Immunoprecipitate
Experimental Factor Type: Immunoprecipitate

Citation(s)

21685892

Submission date

Jul 27, 2011

Last update date

May 15, 2019

Organization

European Bioinformatics Institute

E-mail(s)

miamexpress@ebi.ac.uk

Lab

ArrayExpress